Purification of extracellular lipases from Trichosporon fermentans WU-C12

Jiacong Chen, Susumu Shimura, Kotaro Kirimura, Shoji Usami

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Two types of extracellular lipases (I and II) from Trichosporon fermentans WU-C12 were purified by acetone precipitation and successive chromatographies on Butyl-Toyopearl 650 M, Toyopearl HW-55F and Q-Sepharose FF. The molecular weight of lipase I was 53 kDa by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and 160 kDa by gel filtration, while that of lipase II was 55 kDa by SDS-PAGE and 60 kDa by gel filtration. For the hydrolysis of olive oil, the optimum pH and temperature of both the lipases were 5.5 and 35°C, respectively. The lipases showed stable activities after incubation at 30°C for 24 h in a pH range from 4.0 to 8.0. The thermostability of lipase I for 30 min at a reaction pH of 5.5 was up to 40°C, while that of lipase II under the same conditions was up to 50°C. Both lipases could hydrolyze the 1-, 2-, and 3-positions of triolein, and cleave all three ester bonds, regardless of the position in the triglyceride.

    Original languageEnglish
    Pages (from-to)548-550
    Number of pages3
    JournalJournal of Fermentation and Bioengineering
    Volume77
    Issue number5
    DOIs
    Publication statusPublished - 1994

    Fingerprint

    Trichosporon
    Lipases
    Lipase
    Purification
    Gels
    Gel Chromatography
    Polyacrylamide Gel Electrophoresis
    Electrophoresis
    Triolein
    Polyacrylates
    Acetone
    Sepharose
    Chromatography
    Esters
    Triglycerides
    Olive oil
    Hydrolysis
    Molecular Weight
    Molecular weight
    Temperature

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Purification of extracellular lipases from Trichosporon fermentans WU-C12. / Chen, Jiacong; Shimura, Susumu; Kirimura, Kotaro; Usami, Shoji.

    In: Journal of Fermentation and Bioengineering, Vol. 77, No. 5, 1994, p. 548-550.

    Research output: Contribution to journalArticle

    Chen, Jiacong ; Shimura, Susumu ; Kirimura, Kotaro ; Usami, Shoji. / Purification of extracellular lipases from Trichosporon fermentans WU-C12. In: Journal of Fermentation and Bioengineering. 1994 ; Vol. 77, No. 5. pp. 548-550.
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