Abstract
A cell free protein synthesis approach is extensively used for biochemical and synthetic biology researches. Unlike lysate based cell free systems, the PURE system is reconstituted with individually purified factors essential for transcriptional and translational processes. Hence, the components in the PURE system can be definitely manipulated as per the desired situation. Because of this high controllability, the PURE system has been applied to a wide range of research scene, such as biochemical analysis in reconstructed system, in vitro protein engineering, reconstitution of an artificial cell. We believe that this purified cell-free protein synthesis system become a basal technology to advance synthetic biology.
| Original language | English |
|---|---|
| Pages (from-to) | 158-162 |
| Number of pages | 5 |
| Journal | Current Opinion in Chemical Biology |
| Volume | 22 |
| DOIs | |
| Publication status | Published - 2014 Oct 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
Cite this
Purified cell-free systems as standard parts for synthetic biology. / Matsubayashi, Hideaki; Ueda, Takuya.
In: Current Opinion in Chemical Biology, Vol. 22, 01.10.2014, p. 158-162.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Purified cell-free systems as standard parts for synthetic biology
AU - Matsubayashi, Hideaki
AU - Ueda, Takuya
PY - 2014/10/1
Y1 - 2014/10/1
N2 - A cell free protein synthesis approach is extensively used for biochemical and synthetic biology researches. Unlike lysate based cell free systems, the PURE system is reconstituted with individually purified factors essential for transcriptional and translational processes. Hence, the components in the PURE system can be definitely manipulated as per the desired situation. Because of this high controllability, the PURE system has been applied to a wide range of research scene, such as biochemical analysis in reconstructed system, in vitro protein engineering, reconstitution of an artificial cell. We believe that this purified cell-free protein synthesis system become a basal technology to advance synthetic biology.
AB - A cell free protein synthesis approach is extensively used for biochemical and synthetic biology researches. Unlike lysate based cell free systems, the PURE system is reconstituted with individually purified factors essential for transcriptional and translational processes. Hence, the components in the PURE system can be definitely manipulated as per the desired situation. Because of this high controllability, the PURE system has been applied to a wide range of research scene, such as biochemical analysis in reconstructed system, in vitro protein engineering, reconstitution of an artificial cell. We believe that this purified cell-free protein synthesis system become a basal technology to advance synthetic biology.
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UR - http://www.scopus.com/inward/citedby.url?scp=84908703254&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2014.09.031
DO - 10.1016/j.cbpa.2014.09.031
M3 - Review article
VL - 22
SP - 158
EP - 162
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
SN - 1367-5931
ER -