Pyridine-promoted factor- and energy-free peptide synthesis systems prepared from various organisms including prokaryote, eukaryote, and mitochondria

Takahiko Nojima, Itaru Nitta, Takuya Ueda, Kimitsuna Watanabe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We demonstrate here that ribosomes from not only Escherichia coli and Thermus thermophilus but also yeast and bovine mitochondria catalyze peptide synthesis promoted by a high concentration of pyridine in the absence of soluble protein factors and chemical energy sources, and compare some characteristic features of the reactions among these organisms. Sensitivities against antibiotics, chloramphenicol and cycloheximide, showed the same tendency to those in the in vitro aqueous translation systems of these organisms, suggesting that the basic mechanism for peptide synthesis is the same among these organisms. The optimal concentration of pyridine was centered at 50% for all systems, although the dependencies on the pyridine concentrations and the yields of the products were different from one another. All these systems required Mg2+, and only mitochondrial system showed the extra Mn2+-requirement, which enhanced the yield by several fold. The optimum reaction temperatures coincided closely with the growing temperatures of the organisms except for the mitochondrial system, which showed the highest activity above 80°C. The rationale for these observations remains to be solved.

Original languageEnglish
Pages (from-to)1076-1079
Number of pages4
JournalJournal of biochemistry
Volume119
Issue number6
DOIs
Publication statusPublished - 1996 Jun
Externally publishedYes

Keywords

  • Antibiotics
  • Mn ion
  • Peptide synthesis system
  • Pyridine
  • Ribosomes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Pyridine-promoted factor- and energy-free peptide synthesis systems prepared from various organisms including prokaryote, eukaryote, and mitochondria'. Together they form a unique fingerprint.

  • Cite this