Quantification and localization of erythropoietin-receptor-expressing cells in the liver of Xenopus laevis

Takehito Okui, Yusuke Yamamoto, Shun Maekawa, Kazumichi Nagasawa, Yuka Yonezuka, Youichi Aizawa, Takashi Kato

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Erythropoiesis occurs in the African clawed frog, Xenopus laevis and is mediated by erythropoietin (xlEPO), a primary regulator of this process. Previously, we have shown that the xlEPO receptor (xlEPOR), which is expressed by erythroid progenitors that respond to xlEPO, is found predominantly in the liver. The aim of the present study was to determine the dynamics of erythropoiesis in the livers of normal and anemic X. laevis by identifying the number and precise location of mature and immature erythrocytes. We quantified mature and immature erythrocyte numbers by o-dianisidine staining or immunohistochemistry and investigated the dynamics of erythropoiesis in normal, acute hemolytic and blood-loss states by in vivo cell proliferation assays with 5-bromo-2′-deoxyuridine (BrdU). We detected 0.12×108 xlEPOR+ BrdU+ cells in the liver of the normal X. laevis at 24 h after BrdU injection. Frogs presenting with acute hemolytic anemia and pancytopenia show a 10-fold increase in the number of xlEPOR+/ BrdU+ cells (approximately 1.30×108 cells) in the liver. The xlEPOR+ cells are found predominantly on the inner wall of hepatic sinusoids. Hematopoietic progenitors that undergo slow cell cycling were also observed in the hepatic sinusoids. This study clarifies the rate of production of mature and immature erythrocytes per day in the liver of X. laevis and the way that these cell numbers change in response to anemia.

    Original languageEnglish
    Pages (from-to)153-164
    Number of pages12
    JournalCell and Tissue Research
    Volume353
    Issue number1
    DOIs
    Publication statusPublished - 2013 Jul

    Fingerprint

    Erythropoietin Receptors
    Xenopus laevis
    Liver
    Deoxyuridine
    Erythropoiesis
    Anura
    Dianisidine
    Erythrocytes
    Pancytopenia
    Erythrocyte Count
    Hemolytic Anemia
    Bromodeoxyuridine
    Erythropoietin
    Anemia
    Cell Count
    Immunohistochemistry
    Cell Proliferation
    Staining and Labeling
    Injections

    Keywords

    • Anemia
    • Erythropoiesis
    • Liver
    • Slow cycling cell
    • Xenopus laevis

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine
    • Cell Biology
    • Histology

    Cite this

    Quantification and localization of erythropoietin-receptor-expressing cells in the liver of Xenopus laevis. / Okui, Takehito; Yamamoto, Yusuke; Maekawa, Shun; Nagasawa, Kazumichi; Yonezuka, Yuka; Aizawa, Youichi; Kato, Takashi.

    In: Cell and Tissue Research, Vol. 353, No. 1, 07.2013, p. 153-164.

    Research output: Contribution to journalArticle

    Okui, Takehito ; Yamamoto, Yusuke ; Maekawa, Shun ; Nagasawa, Kazumichi ; Yonezuka, Yuka ; Aizawa, Youichi ; Kato, Takashi. / Quantification and localization of erythropoietin-receptor-expressing cells in the liver of Xenopus laevis. In: Cell and Tissue Research. 2013 ; Vol. 353, No. 1. pp. 153-164.
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