Quantification of genetically modified soybean by quenching probe polymerase chain reaction

Hidenori Tani, Naohiro Noda, Kazutaka Yamada, Shinya Kurata, Satoshi Tsuneda, Akira Hirata, Takahiro Kanagawa

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Quenching probe (QProbe) polymerase chain reaction (PCR) is a simple and cost-effective real-time PCR assay in comparison with other real-time PCR assays such as the TaqMan assay. We used QProbe-PCR to quantify genetically modified (GM) soybean (Roundup Ready soybean). We designed event-specific QProbes for Le1 (soy endogenous gene) and RRS (recombinant gene), and we quantified certified reference materials containing 0.1, 0.5, 1, 2, and 5% GM soybean. The TaqMan assay was also applied to the same samples, and the results were compared. The accuracy of QProbe-PCR was similar to that of TaqMan assay. When GM soybean content was 0.5% or more, the relative standard deviations of QProbe-PCR were less than 20%. QProbe-PCR is sensitive enough to monitor labeling systems and has acceptable levels of accuracy and precision.

    Original languageEnglish
    Pages (from-to)2535-2540
    Number of pages6
    JournalJournal of Agricultural and Food Chemistry
    Volume53
    Issue number7
    DOIs
    Publication statusPublished - 2005 Apr 6

    Fingerprint

    Polymerase chain reaction
    Soybeans
    Quenching
    polymerase chain reaction
    soybeans
    Polymerase Chain Reaction
    Assays
    assays
    glyphosate
    Real-Time Polymerase Chain Reaction
    quantitative polymerase chain reaction
    Genes
    genes
    Labeling
    Costs and Cost Analysis
    monitoring
    sampling
    Costs

    Keywords

    • Fluorescence quenching
    • GMOs
    • QProbe-PCR
    • Quenching probe
    • Real-time PCR
    • Roundup Ready soybean

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences (miscellaneous)
    • Food Science
    • Chemistry (miscellaneous)

    Cite this

    Quantification of genetically modified soybean by quenching probe polymerase chain reaction. / Tani, Hidenori; Noda, Naohiro; Yamada, Kazutaka; Kurata, Shinya; Tsuneda, Satoshi; Hirata, Akira; Kanagawa, Takahiro.

    In: Journal of Agricultural and Food Chemistry, Vol. 53, No. 7, 06.04.2005, p. 2535-2540.

    Research output: Contribution to journalArticle

    Tani, Hidenori ; Noda, Naohiro ; Yamada, Kazutaka ; Kurata, Shinya ; Tsuneda, Satoshi ; Hirata, Akira ; Kanagawa, Takahiro. / Quantification of genetically modified soybean by quenching probe polymerase chain reaction. In: Journal of Agricultural and Food Chemistry. 2005 ; Vol. 53, No. 7. pp. 2535-2540.
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    abstract = "Quenching probe (QProbe) polymerase chain reaction (PCR) is a simple and cost-effective real-time PCR assay in comparison with other real-time PCR assays such as the TaqMan assay. We used QProbe-PCR to quantify genetically modified (GM) soybean (Roundup Ready soybean). We designed event-specific QProbes for Le1 (soy endogenous gene) and RRS (recombinant gene), and we quantified certified reference materials containing 0.1, 0.5, 1, 2, and 5{\%} GM soybean. The TaqMan assay was also applied to the same samples, and the results were compared. The accuracy of QProbe-PCR was similar to that of TaqMan assay. When GM soybean content was 0.5{\%} or more, the relative standard deviations of QProbe-PCR were less than 20{\%}. QProbe-PCR is sensitive enough to monitor labeling systems and has acceptable levels of accuracy and precision.",
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    AU - Noda, Naohiro

    AU - Yamada, Kazutaka

    AU - Kurata, Shinya

    AU - Tsuneda, Satoshi

    AU - Hirata, Akira

    AU - Kanagawa, Takahiro

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    AB - Quenching probe (QProbe) polymerase chain reaction (PCR) is a simple and cost-effective real-time PCR assay in comparison with other real-time PCR assays such as the TaqMan assay. We used QProbe-PCR to quantify genetically modified (GM) soybean (Roundup Ready soybean). We designed event-specific QProbes for Le1 (soy endogenous gene) and RRS (recombinant gene), and we quantified certified reference materials containing 0.1, 0.5, 1, 2, and 5% GM soybean. The TaqMan assay was also applied to the same samples, and the results were compared. The accuracy of QProbe-PCR was similar to that of TaqMan assay. When GM soybean content was 0.5% or more, the relative standard deviations of QProbe-PCR were less than 20%. QProbe-PCR is sensitive enough to monitor labeling systems and has acceptable levels of accuracy and precision.

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