Quantification of genetically modified soybean by quenching probe polymerase chain reaction

Hidenori Tani, Naohiro Noda, Kazutaka Yamada, Shinya Kurata, Satoshi Tsuneda, Akira Hirata, Takahiro Kanagawa

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Quenching probe (QProbe) polymerase chain reaction (PCR) is a simple and cost-effective real-time PCR assay in comparison with other real-time PCR assays such as the TaqMan assay. We used QProbe-PCR to quantify genetically modified (GM) soybean (Roundup Ready soybean). We designed event-specific QProbes for Le1 (soy endogenous gene) and RRS (recombinant gene), and we quantified certified reference materials containing 0.1, 0.5, 1, 2, and 5% GM soybean. The TaqMan assay was also applied to the same samples, and the results were compared. The accuracy of QProbe-PCR was similar to that of TaqMan assay. When GM soybean content was 0.5% or more, the relative standard deviations of QProbe-PCR were less than 20%. QProbe-PCR is sensitive enough to monitor labeling systems and has acceptable levels of accuracy and precision.

Original languageEnglish
Pages (from-to)2535-2540
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number7
DOIs
Publication statusPublished - 2005 Apr 6

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Keywords

  • Fluorescence quenching
  • GMOs
  • QProbe-PCR
  • Quenching probe
  • Real-time PCR
  • Roundup Ready soybean

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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