Quantitative analysis of amoA mRNA expression as a new biomarker of ammonia oxidation activities in a complex microbial community

Yoshiteru Aoi, Y. Masaki, Satoshi Tsuneda, A. Hirata

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Aims: To quantitatively analyse the changes to amoA mRNA (ammonia mono-oxygenase encoding mRNA) profiles in response to a change in ammonia oxidation activity in a complex microbial community. Methods and Results: The amoA mRNA levels in both a batch-mode incubation and a continuously fed nitrification reactor were determined by real-time reverse transcription-PCR analysis. The amoA mRNA level changed rapidly in response to the change in environmental conditions which affect ammonia oxidation activity. Conclusion: An increase in amoA mRNA level can be detected within 1-2 h in response to an initiation of cell activity whereas a decrease in amoA mRNA level is detected within 24 h in response to a cessation of activity. Significance and Impact of the Study: amoA mRNA, which shows sensitive response to ammonia oxidation activity, can be used as a biomarker of ammonia oxidation activity in wastewater treatment processes where many bacterial species exist.

    Original languageEnglish
    Pages (from-to)477-482
    Number of pages6
    JournalLetters in Applied Microbiology
    Volume39
    Issue number6
    DOIs
    Publication statusPublished - 2004

    Fingerprint

    Biomarkers
    Ammonia
    quantitative analysis
    biomarker
    microbial community
    ammonia
    oxidation
    Oxidation
    Messenger RNA
    Chemical analysis
    Bacterial Physiological Phenomena
    Nitrification
    nitrification
    Transcription
    incubation
    environmental conditions
    Waste Water
    Wastewater treatment
    Reverse Transcription
    Polymerase Chain Reaction

    Keywords

    • Ammonia oxidizing bacteria
    • mRNA
    • Nitrification
    • Real-time reverse transcription-PCR
    • rRNA
    • Wastewater treatment process

    ASJC Scopus subject areas

    • Applied Microbiology and Biotechnology
    • Water Science and Technology
    • Fluid Flow and Transfer Processes
    • Microbiology
    • Ecology

    Cite this

    Quantitative analysis of amoA mRNA expression as a new biomarker of ammonia oxidation activities in a complex microbial community. / Aoi, Yoshiteru; Masaki, Y.; Tsuneda, Satoshi; Hirata, A.

    In: Letters in Applied Microbiology, Vol. 39, No. 6, 2004, p. 477-482.

    Research output: Contribution to journalArticle

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