Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: an experimental study on the anti-adhesion effect of green-tea catechins

Rumi Sakamoto, Eisuke Kakinuma, Kentaro Masuda, Yuko Takeuchi, Kosaku Ito, Kentaro Iketaki, Takahisa Matsuzaki, Seiichiro Nakabayashi, Hiroshi Y. Yoshikawa, Hideaki Yamamoto, Yuko Sato, Takashi Tanii

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    The main constituent of green tea, (−)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (−)-Epicatechin-3-O-gallate (ECG) and (−)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion—suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells—and validates the use of OMT as a tool for screening cancer cell adhesion.

    Original languageEnglish
    Pages (from-to)1-7
    Number of pages7
    JournalIn Vitro Cellular and Developmental Biology - Animal
    DOIs
    Publication statusAccepted/In press - 2016 Jun 1

    Fingerprint

    Catechin
    Tea
    Cell Adhesion
    Neoplasms
    Interference Microscopy
    Pancreatic Neoplasms
    Early Detection of Cancer
    Pancreas
    epigallocatechin gallate

    Keywords

    • Anti-adhesion effect
    • Cell adhesion
    • Green-tea catechin
    • Organosilane monolayer template
    • Reflection interference contrast microscopy

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology

    Cite this

    Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates : an experimental study on the anti-adhesion effect of green-tea catechins. / Sakamoto, Rumi; Kakinuma, Eisuke; Masuda, Kentaro; Takeuchi, Yuko; Ito, Kosaku; Iketaki, Kentaro; Matsuzaki, Takahisa; Nakabayashi, Seiichiro; Yoshikawa, Hiroshi Y.; Yamamoto, Hideaki; Sato, Yuko; Tanii, Takashi.

    In: In Vitro Cellular and Developmental Biology - Animal, 01.06.2016, p. 1-7.

    Research output: Contribution to journalArticle

    Sakamoto, Rumi ; Kakinuma, Eisuke ; Masuda, Kentaro ; Takeuchi, Yuko ; Ito, Kosaku ; Iketaki, Kentaro ; Matsuzaki, Takahisa ; Nakabayashi, Seiichiro ; Yoshikawa, Hiroshi Y. ; Yamamoto, Hideaki ; Sato, Yuko ; Tanii, Takashi. / Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates : an experimental study on the anti-adhesion effect of green-tea catechins. In: In Vitro Cellular and Developmental Biology - Animal. 2016 ; pp. 1-7.
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    abstract = "The main constituent of green tea, (−)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (−)-Epicatechin-3-O-gallate (ECG) and (−)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion—suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells—and validates the use of OMT as a tool for screening cancer cell adhesion.",
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    AU - Sakamoto, Rumi

    AU - Kakinuma, Eisuke

    AU - Masuda, Kentaro

    AU - Takeuchi, Yuko

    AU - Ito, Kosaku

    AU - Iketaki, Kentaro

    AU - Matsuzaki, Takahisa

    AU - Nakabayashi, Seiichiro

    AU - Yoshikawa, Hiroshi Y.

    AU - Yamamoto, Hideaki

    AU - Sato, Yuko

    AU - Tanii, Takashi

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