Abstract
The main constituent of green tea, (−)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (−)-Epicatechin-3-O-gallate (ECG) and (−)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion—suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells—and validates the use of OMT as a tool for screening cancer cell adhesion.
| Original language | English |
|---|---|
| Pages (from-to) | 1-7 |
| Number of pages | 7 |
| Journal | In Vitro Cellular and Developmental Biology - Animal |
| DOIs | |
| Publication status | Accepted/In press - 2016 Jun 1 |
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Keywords
- Anti-adhesion effect
- Cell adhesion
- Green-tea catechin
- Organosilane monolayer template
- Reflection interference contrast microscopy
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates : an experimental study on the anti-adhesion effect of green-tea catechins. / Sakamoto, Rumi; Kakinuma, Eisuke; Masuda, Kentaro; Takeuchi, Yuko; Ito, Kosaku; Iketaki, Kentaro; Matsuzaki, Takahisa; Nakabayashi, Seiichiro; Yoshikawa, Hiroshi Y.; Yamamoto, Hideaki; Sato, Yuko; Tanii, Takashi.
In: In Vitro Cellular and Developmental Biology - Animal, 01.06.2016, p. 1-7.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates
T2 - an experimental study on the anti-adhesion effect of green-tea catechins
AU - Sakamoto, Rumi
AU - Kakinuma, Eisuke
AU - Masuda, Kentaro
AU - Takeuchi, Yuko
AU - Ito, Kosaku
AU - Iketaki, Kentaro
AU - Matsuzaki, Takahisa
AU - Nakabayashi, Seiichiro
AU - Yoshikawa, Hiroshi Y.
AU - Yamamoto, Hideaki
AU - Sato, Yuko
AU - Tanii, Takashi
PY - 2016/6/1
Y1 - 2016/6/1
N2 - The main constituent of green tea, (−)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (−)-Epicatechin-3-O-gallate (ECG) and (−)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion—suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells—and validates the use of OMT as a tool for screening cancer cell adhesion.
AB - The main constituent of green tea, (−)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (−)-Epicatechin-3-O-gallate (ECG) and (−)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion—suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells—and validates the use of OMT as a tool for screening cancer cell adhesion.
KW - Anti-adhesion effect
KW - Cell adhesion
KW - Green-tea catechin
KW - Organosilane monolayer template
KW - Reflection interference contrast microscopy
UR - http://www.scopus.com/inward/record.url?scp=84973177472&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973177472&partnerID=8YFLogxK
U2 - 10.1007/s11626-016-0049-6
DO - 10.1007/s11626-016-0049-6
M3 - Article
C2 - 27251159
AN - SCOPUS:84973177472
SP - 1
EP - 7
JO - In Vitro Cellular and Developmental Biology - Animal
JF - In Vitro Cellular and Developmental Biology - Animal
SN - 1071-2690
ER -