Quantitative detection of immunoreaction using magnetite nanoparticles and Raman scattering spectroscopy

Sho Hideshima, Masahiro Yanagisawa, Atsushi Arakaki, Tadashi Matsunaga, Tetsuya Osaka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We developed a new quantitative detection method for an immunoreaction combining magnetite (Fe3O4) nanoparticle labeling with confocal Raman spectrometry. Magnetite nanoparticles are transparent, Raman active, and easily can be controlled easily by magnets in bimolecular reactions. Human Chorionic Gonadtropin (hCG) was selected as a target molecule for this investigation. Sandwich immunoreaction was performed on a dot-patterned substrate using two anti-hCG antibodies, which were the antibody immobilized on the substrate and the biotinylated antibody. The immunoreaction was microscopically visualized by reacting streptavidin-modified magnetite nanoparticles with the biotinylated antibody on the substrate. The adsorbed magnetite nanoparticles on the dot pattern were detected by Raman intensity imaging. A good linear relation between the integrated Raman intensity spectra at 220 cm-1 and hCG concentration was gained. This method may be applied toward the detection of other target molecules in the fields of biotechnology and biomedicine.

Original languageEnglish
Pages (from-to)142-146
Number of pages5
Journale-Journal of Surface Science and Nanotechnology
Volume6
DOIs
Publication statusPublished - 2008 May 31

Fingerprint

Magnetite Nanoparticles
Magnetite nanoparticles
Raman Spectrum Analysis
antibodies
Antibodies
magnetite
Raman scattering
Spectroscopy
Raman spectra
nanoparticles
Substrates
scattering
Immobilized Antibodies
spectroscopy
Molecules
Streptavidin
Biotechnology
Labeling
Spectrometry
biotechnology

Keywords

  • Biosensing and devices
  • Immunoreaction
  • Iron oxide
  • Magnetite nanoparticle
  • Raman scattering spectroscopy
  • Self-assembly

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Materials Science (miscellaneous)

Cite this

Quantitative detection of immunoreaction using magnetite nanoparticles and Raman scattering spectroscopy. / Hideshima, Sho; Yanagisawa, Masahiro; Arakaki, Atsushi; Matsunaga, Tadashi; Osaka, Tetsuya.

In: e-Journal of Surface Science and Nanotechnology, Vol. 6, 31.05.2008, p. 142-146.

Research output: Contribution to journalArticle

Hideshima, Sho ; Yanagisawa, Masahiro ; Arakaki, Atsushi ; Matsunaga, Tadashi ; Osaka, Tetsuya. / Quantitative detection of immunoreaction using magnetite nanoparticles and Raman scattering spectroscopy. In: e-Journal of Surface Science and Nanotechnology. 2008 ; Vol. 6. pp. 142-146.
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