Quantitative determination of branched-chain amino acids in human plasma using pressure-driven liquid chromatography with pillar array columns

Y. Song, K. Takatsuki, M. Isokawa, T. Sekiguchi, J. Mizuno, T. Funatsu, S. Shoji, M. Tsunoda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have succeeded for the first time to quantify biological compounds in human plasma using pillar array columns. A fast and quantitative method for the determination of branched-chain amino acids (BCAAs) in human plasma using pressure-driven liquid chromatography on a chip was developed. BCAAs were firstly derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and then analysis of NBD-BCAAs was optimized to be finished in 100 s. With the help of an internal standard, the concentrations of BCAAs in human plasma samples were quantitatively determined. The developed method should be a useful tool for fast diagnosis in clinical field.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1794-1796
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Keywords

  • Fluorescence
  • Internal standard
  • NBD-F

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Song, Y., Takatsuki, K., Isokawa, M., Sekiguchi, T., Mizuno, J., Funatsu, T., Shoji, S., & Tsunoda, M. (2013). Quantitative determination of branched-chain amino acids in human plasma using pressure-driven liquid chromatography with pillar array columns. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1794-1796). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3). Chemical and Biological Microsystems Society.