Quantitative evaluation of a gold-nanoparticle labeling method for detecting target DNAs on DNA microarrays

Hyonchol Kim, Hiroyuki Takei, Kenji Yasuda

Research output: Contribution to journalLetter

16 Citations (Scopus)

Abstract

The detection sensitivity obtained when combining electron-microscopy counting with gold-nanoparticle labeling of target nucleic acids complementary to probe DNA microarrays with the detection sensitivity obtained when using optical-microscopy measurement of conventional fluorescent labeling. Target DNAs were reacted with probe DNAs on DNA microarrays on which micro-columns had been etched so the target DNA could be agitated. The targets were reacted with the other probe DNA immobilized onto the surface of the nanoparticles. The labeled target DNAs were measured by using field-emission scanning electron microscopy (FE-SEM) imaging and counting the nanoparticles. The detection sensitivity of nanoparticle-labeling measurement was 1000 times that of fluorescent-labeling measurement, and the S/N ratio of nanoparticle-labeling measurement was 100 times that of fluorescent-labeling measurement. The results indicate that the nanoparticle-labeling method using FE-SEM counting is sensitive enough that it has a possibility for measuring targets expressed in a cell without amplification such as polymerase chain reaction (PCR).

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume144
Issue number1
DOIs
Publication statusPublished - 2010 Jan 29
Externally publishedYes

Fingerprint

Microarrays
Gold
Labeling
marking
DNA
deoxyribonucleic acid
gold
Nanoparticles
nanoparticles
evaluation
DNA Probes
counting
Field emission
probes
field emission
Immobilized Nucleic Acids
polymerase chain reaction
Scanning electron microscopy
scanning electron microscopy
Polymerase chain reaction

Keywords

  • Agitation
  • DNA microarray
  • Field-emission scanning electron microscopy
  • Gold nanoparticle
  • Labeling

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Quantitative evaluation of a gold-nanoparticle labeling method for detecting target DNAs on DNA microarrays. / Kim, Hyonchol; Takei, Hiroyuki; Yasuda, Kenji.

In: Sensors and Actuators, B: Chemical, Vol. 144, No. 1, 29.01.2010, p. 6-10.

Research output: Contribution to journalLetter

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N2 - The detection sensitivity obtained when combining electron-microscopy counting with gold-nanoparticle labeling of target nucleic acids complementary to probe DNA microarrays with the detection sensitivity obtained when using optical-microscopy measurement of conventional fluorescent labeling. Target DNAs were reacted with probe DNAs on DNA microarrays on which micro-columns had been etched so the target DNA could be agitated. The targets were reacted with the other probe DNA immobilized onto the surface of the nanoparticles. The labeled target DNAs were measured by using field-emission scanning electron microscopy (FE-SEM) imaging and counting the nanoparticles. The detection sensitivity of nanoparticle-labeling measurement was 1000 times that of fluorescent-labeling measurement, and the S/N ratio of nanoparticle-labeling measurement was 100 times that of fluorescent-labeling measurement. The results indicate that the nanoparticle-labeling method using FE-SEM counting is sensitive enough that it has a possibility for measuring targets expressed in a cell without amplification such as polymerase chain reaction (PCR).

AB - The detection sensitivity obtained when combining electron-microscopy counting with gold-nanoparticle labeling of target nucleic acids complementary to probe DNA microarrays with the detection sensitivity obtained when using optical-microscopy measurement of conventional fluorescent labeling. Target DNAs were reacted with probe DNAs on DNA microarrays on which micro-columns had been etched so the target DNA could be agitated. The targets were reacted with the other probe DNA immobilized onto the surface of the nanoparticles. The labeled target DNAs were measured by using field-emission scanning electron microscopy (FE-SEM) imaging and counting the nanoparticles. The detection sensitivity of nanoparticle-labeling measurement was 1000 times that of fluorescent-labeling measurement, and the S/N ratio of nanoparticle-labeling measurement was 100 times that of fluorescent-labeling measurement. The results indicate that the nanoparticle-labeling method using FE-SEM counting is sensitive enough that it has a possibility for measuring targets expressed in a cell without amplification such as polymerase chain reaction (PCR).

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