Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy

Takahisa Matsuzaki, Kosaku Ito, Kentaro Masuda, Eisuke Kakinuma, Rumi Sakamoto, Kentaro Iketaki, Hideaki Yamamoto, Masami Suganuma, Naritaka Kobayashi, Seiichiro Nakabayashi, Takashi Tanii, Hiroshi Y. Yoshikawa

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Adhesion of cancer cells with different metastatic potential and anticancer drug resistance has been quantitatively evaluated by using self-assembled monolayer (SAM)-patterned substrates and reflection interference contrast microscopy (RICM). Cell-adhesive SAM spots with optimized diameter could prevent cell-cell adhesion and thus allowed the systematic evaluation of statistically reliable numbers of contact area between single cancer cells and substrates by RICM. The statistical image analysis revealed that highly metastatic mouse melanoma cells showed larger contact area than lowly metastatic cells. We also found that both cancer cell types exhibited distinct transition from the "strong" to "weak" adhesion states with increase in the concentration of (-)-epigallocatechin gallate (EGCG), which is known to exhibit cancer preventive activity. Mathematical analysis of the adhesion transition revealed that adhesion of the highly metastatic mouse melanoma cells showed more EGCG tolerance than that of lowly metastatic cells. Moreover, time-lapse RICM observation revealed that EGCG weakened cancer cell adhesion in a stepwise manner, probably via focal adhesion complex. These results clearly indicate that contact area can be used as a quantitative measure for the determination of cancer phenotypes and their drug resistance, which will provide physical insights into the mechanism of cancer metastasis and cancer prevention.

    Original languageEnglish
    Pages (from-to)1221-1227
    Number of pages7
    JournalJournal of Physical Chemistry B
    Volume120
    Issue number7
    DOIs
    Publication statusPublished - 2016 Feb 25

    Fingerprint

    Cell adhesion
    Self assembled monolayers
    Microscopic examination
    adhesion
    Adhesion
    cancer
    microscopy
    interference
    evaluation
    Substrates
    gallates
    Cells
    cells
    mice
    drugs
    Pharmaceutical Preparations
    Image analysis
    Adhesives
    phenotype
    applications of mathematics

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry
    • Materials Chemistry
    • Surfaces, Coatings and Films

    Cite this

    Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy. / Matsuzaki, Takahisa; Ito, Kosaku; Masuda, Kentaro; Kakinuma, Eisuke; Sakamoto, Rumi; Iketaki, Kentaro; Yamamoto, Hideaki; Suganuma, Masami; Kobayashi, Naritaka; Nakabayashi, Seiichiro; Tanii, Takashi; Yoshikawa, Hiroshi Y.

    In: Journal of Physical Chemistry B, Vol. 120, No. 7, 25.02.2016, p. 1221-1227.

    Research output: Contribution to journalArticle

    Matsuzaki, T, Ito, K, Masuda, K, Kakinuma, E, Sakamoto, R, Iketaki, K, Yamamoto, H, Suganuma, M, Kobayashi, N, Nakabayashi, S, Tanii, T & Yoshikawa, HY 2016, 'Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy', Journal of Physical Chemistry B, vol. 120, no. 7, pp. 1221-1227. https://doi.org/10.1021/acs.jpcb.5b11870
    Matsuzaki, Takahisa ; Ito, Kosaku ; Masuda, Kentaro ; Kakinuma, Eisuke ; Sakamoto, Rumi ; Iketaki, Kentaro ; Yamamoto, Hideaki ; Suganuma, Masami ; Kobayashi, Naritaka ; Nakabayashi, Seiichiro ; Tanii, Takashi ; Yoshikawa, Hiroshi Y. / Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy. In: Journal of Physical Chemistry B. 2016 ; Vol. 120, No. 7. pp. 1221-1227.
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