Abstract
Cell-to-cell communication is considered to underlie the coordinated behavior and the multicellularity of cell group class, which cannot be explained only by the knowledge of lower class of life system from molecule to individual cell, because they are determined by at least two different ways: diffusible chemical signals and their direct physical contacts. We show in this paper a new method of individual-cell-based cell observation that can estimate the role of cell-to-cell communication, diffusible chemical signals, and physical contacts as separated properties, by applying an on-chip individual-cell-based cultivation system. The exchange of stationary phase medium on isolated individual Escherichia coli from exponential phase medium and the control of physical contacts indicated that the cell-to-cell direct contact did not affect the growth rate; only the communication through diffusible signals affects the growth rates as Hill's equation manner.
Original language | English |
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Pages (from-to) | 1130-1138 |
Number of pages | 9 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 349 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2006 Oct 27 |
Externally published | Yes |
Keywords
- Cell-to-cell communication
- Diffusible signals
- Growth rate
- Isolated individual Escherichia coli
- Physical contact
- Single cell cultivation
- Stationary phase
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology