Abstract
Cell-to-cell communication is considered to underlie the coordinated behavior and the multicellularity of cell group class, which cannot be explained only by the knowledge of lower class of life system from molecule to individual cell, because they are determined by at least two different ways: diffusible chemical signals and their direct physical contacts. We show in this paper a new method of individual-cell-based cell observation that can estimate the role of cell-to-cell communication, diffusible chemical signals, and physical contacts as separated properties, by applying an on-chip individual-cell-based cultivation system. The exchange of stationary phase medium on isolated individual Escherichia coli from exponential phase medium and the control of physical contacts indicated that the cell-to-cell direct contact did not affect the growth rate; only the communication through diffusible signals affects the growth rates as Hill's equation manner.
| Original language | English |
|---|---|
| Pages (from-to) | 1130-1138 |
| Number of pages | 9 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 349 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2006 Oct 27 |
| Externally published | Yes |
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Keywords
- Cell-to-cell communication
- Diffusible signals
- Growth rate
- Isolated individual Escherichia coli
- Physical contact
- Single cell cultivation
- Stationary phase
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Quantitative evaluation of cell-to-cell communication effects in cell group class using on-chip individual-cell-based cultivation system. / Wakamoto, Yuichi; Yasuda, Kenji.
In: Biochemical and Biophysical Research Communications, Vol. 349, No. 3, 27.10.2006, p. 1130-1138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative evaluation of cell-to-cell communication effects in cell group class using on-chip individual-cell-based cultivation system
AU - Wakamoto, Yuichi
AU - Yasuda, Kenji
PY - 2006/10/27
Y1 - 2006/10/27
N2 - Cell-to-cell communication is considered to underlie the coordinated behavior and the multicellularity of cell group class, which cannot be explained only by the knowledge of lower class of life system from molecule to individual cell, because they are determined by at least two different ways: diffusible chemical signals and their direct physical contacts. We show in this paper a new method of individual-cell-based cell observation that can estimate the role of cell-to-cell communication, diffusible chemical signals, and physical contacts as separated properties, by applying an on-chip individual-cell-based cultivation system. The exchange of stationary phase medium on isolated individual Escherichia coli from exponential phase medium and the control of physical contacts indicated that the cell-to-cell direct contact did not affect the growth rate; only the communication through diffusible signals affects the growth rates as Hill's equation manner.
AB - Cell-to-cell communication is considered to underlie the coordinated behavior and the multicellularity of cell group class, which cannot be explained only by the knowledge of lower class of life system from molecule to individual cell, because they are determined by at least two different ways: diffusible chemical signals and their direct physical contacts. We show in this paper a new method of individual-cell-based cell observation that can estimate the role of cell-to-cell communication, diffusible chemical signals, and physical contacts as separated properties, by applying an on-chip individual-cell-based cultivation system. The exchange of stationary phase medium on isolated individual Escherichia coli from exponential phase medium and the control of physical contacts indicated that the cell-to-cell direct contact did not affect the growth rate; only the communication through diffusible signals affects the growth rates as Hill's equation manner.
KW - Cell-to-cell communication
KW - Diffusible signals
KW - Growth rate
KW - Isolated individual Escherichia coli
KW - Physical contact
KW - Single cell cultivation
KW - Stationary phase
UR - http://www.scopus.com/inward/record.url?scp=33748616839&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748616839&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.08.149
DO - 10.1016/j.bbrc.2006.08.149
M3 - Article
VL - 349
SP - 1130
EP - 1138
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -