Quantitative evaluation of cell-to-cell communication effects in cell group class using on-chip individual-cell-based cultivation system

Yuichi Wakamoto, Kenji Yasuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cell-to-cell communication is considered to underlie the coordinated behavior and the multicellularity of cell group class, which cannot be explained only by the knowledge of lower class of life system from molecule to individual cell, because they are determined by at least two different ways: diffusible chemical signals and their direct physical contacts. We show in this paper a new method of individual-cell-based cell observation that can estimate the role of cell-to-cell communication, diffusible chemical signals, and physical contacts as separated properties, by applying an on-chip individual-cell-based cultivation system. The exchange of stationary phase medium on isolated individual Escherichia coli from exponential phase medium and the control of physical contacts indicated that the cell-to-cell direct contact did not affect the growth rate; only the communication through diffusible signals affects the growth rates as Hill's equation manner.

Original languageEnglish
Pages (from-to)1130-1138
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume349
Issue number3
DOIs
Publication statusPublished - 2006 Oct 27
Externally publishedYes

Fingerprint

Cell Communication
Communication
Escherichia coli
Molecules
Growth
Observation

Keywords

  • Cell-to-cell communication
  • Diffusible signals
  • Growth rate
  • Isolated individual Escherichia coli
  • Physical contact
  • Single cell cultivation
  • Stationary phase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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