Quantitative evaluation of closed-loop-shaped cardiomyocyte network by using ring-shaped electrode

Fumimasa Nomura, Tomoyuki Kaneko, Tomoyo Hamada, Akihiro Hattori, Kenji Yasuda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Re-entry of excitation in the heart is one of the abnormal phenomena that causes lethal arrhythmia and is thought to be induced by the looped structure of the excitation conduction pathway. To evaluate the geometrical pattern dependence of electrophysiological results, we fabricated three models of cardiomyocyte networks and compared their beating frequencies (BFs), amplitudes of a depolarization peak, and field potential durations (FPDs). The set of different closed-loop-shaped network models from 3 to 8mm in length showed the same BFs, amplitudes, and FPDs independent of their loop lengths, whereas the BFs and FPDs of 60 μm small clusters, and the FPDs of the 2mm open-line-shaped network model were different from those of a closed-loop-shaped network model. These results indicate that the mm order larger size of clusters might create lower BFs, and the closed-loop-shaped model may generate longer FPDs. They also suggest the importance of spatial arrangement control of the cardoimyocyte community for reproducible measurement of electrophysiological properties of cardiomyocytes, especially control of the closedloop formation, which might change the waveforms of FPDs depending on the difference in the geometry and conduction pathway of the cell network.

Original languageEnglish
Article number06FK06
JournalJapanese Journal of Applied Physics
Volume51
Issue number6 PART 2
DOIs
Publication statusPublished - 2012 Jun
Externally publishedYes

Fingerprint

potential fields
Electrodes
electrodes
evaluation
rings
arrhythmia
Reentry
Depolarization
conduction
reentry
depolarization
excitation
waveforms
Geometry
low frequencies
causes
geometry
cells

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Quantitative evaluation of closed-loop-shaped cardiomyocyte network by using ring-shaped electrode. / Nomura, Fumimasa; Kaneko, Tomoyuki; Hamada, Tomoyo; Hattori, Akihiro; Yasuda, Kenji.

In: Japanese Journal of Applied Physics, Vol. 51, No. 6 PART 2, 06FK06, 06.2012.

Research output: Contribution to journalArticle

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