Radiation-induced graft polymerization is the key to develop high- performance functional materials for protein purification

Kyoichi Saito, Satoshi Tsuneda, Min Kim, Noboru Kubota, Kazuyuki Sugita, Takanobu Sugo

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    We have described a preparation scheme for immobilizing polymer chains at a uniformly high density onto a microfiltration membrane. Highly efficient protein recovery was demonstrated by the results of the determination of breakthrough and elution curves. The three requirements of high rate, high capacity, and repeated use for the protein recovery were satisfied by ensuring the occurrence of convection, multilayer binding, and hydrophilization, respectively. In addition, easy scale-up to fabrication of a membrane module was verified on a small scale.

    Original languageEnglish
    Pages (from-to)517-525
    Number of pages9
    JournalRadiation Physics and Chemistry
    Volume54
    Issue number5
    DOIs
    Publication statusPublished - 1999 May

    Fingerprint

    purification
    polymerization
    recovery
    membranes
    proteins
    elution
    radiation
    convection
    modules
    occurrences
    requirements
    preparation
    fabrication
    polymers
    curves

    ASJC Scopus subject areas

    • Radiation

    Cite this

    Radiation-induced graft polymerization is the key to develop high- performance functional materials for protein purification. / Saito, Kyoichi; Tsuneda, Satoshi; Kim, Min; Kubota, Noboru; Sugita, Kazuyuki; Sugo, Takanobu.

    In: Radiation Physics and Chemistry, Vol. 54, No. 5, 05.1999, p. 517-525.

    Research output: Contribution to journalArticle

    Saito, Kyoichi ; Tsuneda, Satoshi ; Kim, Min ; Kubota, Noboru ; Sugita, Kazuyuki ; Sugo, Takanobu. / Radiation-induced graft polymerization is the key to develop high- performance functional materials for protein purification. In: Radiation Physics and Chemistry. 1999 ; Vol. 54, No. 5. pp. 517-525.
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    AU - Sugita, Kazuyuki

    AU - Sugo, Takanobu

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