Raman imaging of the diverse states of the filamentous cyanobacteria

J. Ishihara, M. Tachikawa, A. Mochizuki, Y. Sako, Hideo Iwasaki, S. Morita

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    The objective of our research was to predict cell fates of a multicellular system, accompanied by cellular differentiation. To fulfill this objective, we sought to distinguish the differentiated and undifferentiated cells of filamentous cyanobacteria (Anabaena sp. PCC 7120) using Raman imaging. This technique indicated Raman bands of the cellular system, in which several bands were assigned to vibrations of β-carotene and scytonemin. We applied principal component analysis (PCA) to the Raman spectra to determine the PC1 and PC2 loading plots and their scores. The data points obtained for heterocyst tended to converge along the bottom of the scatterplot whereas those for vegetative cells were more widely distributed in the PC plane. This indicates that the chemical compositions of a heterocyst were relatively stable. As vegetative cells are capable of proliferation or differentiation, they may transit and exist in several states including the pseudo-differentiated state. The results suggest that the chemical compositions of a vegetative cell fluctuated according to its cellular condition. In conclusion, the results of Raman imaging indicate that the diverse states of vegetative cells are localized in a specific state through differentiation.

    Original languageEnglish
    Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
    Volume8879
    DOIs
    Publication statusPublished - 2013
    EventSPIE Nano-Bio Sensing, Imaging, and Spectroscopy - Jeju
    Duration: 2013 Feb 202013 Feb 23

    Other

    OtherSPIE Nano-Bio Sensing, Imaging, and Spectroscopy
    CityJeju
    Period13/2/2013/2/23

    Fingerprint

    Cyanobacteria
    Raman
    Imaging
    Imaging techniques
    Cell
    Carotenoids
    cells
    Chemical analysis
    Principal component analysis
    Raman scattering
    chemical composition
    anabaena
    carotene
    Raman Spectra
    Cellular Systems
    transit
    principal components analysis
    Proliferation
    Principal Component Analysis
    Vibration

    Keywords

    • Cyanobacteria
    • differentiation
    • heterocyst
    • Raman imaging
    • Raman microscope
    • vegetative cell

    ASJC Scopus subject areas

    • Applied Mathematics
    • Computer Science Applications
    • Electrical and Electronic Engineering
    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics

    Cite this

    Ishihara, J., Tachikawa, M., Mochizuki, A., Sako, Y., Iwasaki, H., & Morita, S. (2013). Raman imaging of the diverse states of the filamentous cyanobacteria. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 8879). [88790V] https://doi.org/10.1117/12.2018694

    Raman imaging of the diverse states of the filamentous cyanobacteria. / Ishihara, J.; Tachikawa, M.; Mochizuki, A.; Sako, Y.; Iwasaki, Hideo; Morita, S.

    Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8879 2013. 88790V.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Ishihara, J, Tachikawa, M, Mochizuki, A, Sako, Y, Iwasaki, H & Morita, S 2013, Raman imaging of the diverse states of the filamentous cyanobacteria. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 8879, 88790V, SPIE Nano-Bio Sensing, Imaging, and Spectroscopy, Jeju, 13/2/20. https://doi.org/10.1117/12.2018694
    Ishihara J, Tachikawa M, Mochizuki A, Sako Y, Iwasaki H, Morita S. Raman imaging of the diverse states of the filamentous cyanobacteria. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8879. 2013. 88790V https://doi.org/10.1117/12.2018694
    Ishihara, J. ; Tachikawa, M. ; Mochizuki, A. ; Sako, Y. ; Iwasaki, Hideo ; Morita, S. / Raman imaging of the diverse states of the filamentous cyanobacteria. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8879 2013.
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