Rapid and selective discrimination of gram-positive and gram-negative bacteria by boronic acid-modified poly(amidoamine) dendrimer

Yuji Tsuchido, Ryosuke Horiuchi, Takeshi Hashimoto, Kanako Ishihara, Nobuyuki Kanzawa, Takashi Hayashita

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

There is an urgent need to develop a rapid and selective method for the detection of bacteria because delayed diagnosis and the overuse of antibiotics have triggered drug resistance in bacteria. To this end, we prepared boronic acid-modified poly(amidoamine) generation 4 (B-PAMAM(G4)) dendrimer as cross-linking molecules that form aggregates with bacteria. Within 5 min of adding B-PAMAM(G4) dendrimer solution to a bacterial suspension, large aggregates were observed. Interestingly, the aggregate formation with various bacteria was pH-dependent. In basic pH, both Gram-positive and Gram-negative bacteria formed aggregates, but in neutral pH, only Gram-positive bacteria formed aggregates. We revealed that this bacteria-selective aggregation involved the bacterial surface recognition of the phenylboronic acid moiety of B-PAMAM(G4) dendrimer. In addition, we demonstrated that the spherical structure of B-PAMAM(G4) was one of the important factors for the formation of large aggregates. The aggregation was also observed in the presence of ≤10 mM fructose. B-PAMAM(G4) dendrimer is expected to be a powerful tool for the rapid and selective discrimination between Gram-positive and Gram-negative bacteria.

Original languageEnglish
Pages (from-to)3929-3935
Number of pages7
JournalAnalytical chemistry
Volume91
Issue number6
DOIs
Publication statusPublished - 2019 Mar 19
Externally publishedYes

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ASJC Scopus subject areas

  • Analytical Chemistry

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