Rapid and sensitive detection of 17β-estradiol in environmental water using automated immunoassay system with bacterial magnetic particles

Tsuyoshi Tanaka, Hajime Takeda, Fumiko Ueki, Kimimichi Obata, Hideji Tajima, Haruko Takeyama, Yasuhiro Goda, Shigeru Fujimoto, Tadashi Matsunaga

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66 Citations (Scopus)

Abstract

A fully automated immunoassay of 17β-estradiol (E2) was performed using anti-E2 monoclonal antibody immobilized on bacterial magnetic particles (AntiE2-BMPs) and alkaline phosphatase-conjugated E2 (ALP-E2). E2 concentration in environmental water samples was evaluated by decrease in luminescence based on competitive reaction. A linear correlation between the luminescence intensity and E2 concentration was obtained between 0.5 and 5ppb. The minimum detectable concentration of E2 was 20ppt. All measurement steps were done within 0.5h. The analysis of environmental water samples by a commercially available ELISA kit and the BMP-based immunoassay gave good correlation plots with a correlation efficient of 0.992. These results suggest that the fully automated system using the BMP-based immunoassay has some advantages in the high rapidity and sensitivity of the measurement. This system will enable us to determine low E2 concentrations without sample condensation.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalJournal of Biotechnology
Volume108
Issue number2
DOIs
Publication statusPublished - 2004 Mar 4

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Keywords

  • 17β-Estradiol (E2)
  • Anti-E2 monoclonal antibody
  • Bacterial magnetic particles (BMPs)
  • Fully automated immunoassay system

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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