Rapid antibiotic efficacy screening with aluminum oxide nanoporous membrane filter-chip and optical detection system

Pei Hsiang Tsou, Harini Sreenivasappa, Sungmin Hong, Masayuki Yasuike, Hiroshi Miyamoto, Keiyo Nakano, Takeyuki Misawa, Jun Kameoka*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

We have developed a filter-chip and optical detection system for rapid antibiotic efficacy screening. The filter-chip consisted of a 1-mL reservoir and an anodic aluminum oxide (AAO) nanoporous membrane. Sample solution with liquid growth media, bacteria, and antibiotics was incubated in the reservoir for a specific period of time. The number of live bacteria on the surface of membrane was counted after the incubation with antibiotics and filtration. Using this biosensing system, we have demonstrated a 1-h antibiotic screening for patients' clinical samples, significantly faster than the conventional antibiotic susceptibility tests that typically take more than 24. h. This rapid screening nature makes the filter-chip and detection system ideal for tailoring antibiotic treatment to individual patients by reducing the microbial antibiotic resistance, and improving the survival rate for patients suffering from postoperative infections.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number1
DOIs
Publication statusPublished - 2010 Sep
Externally publishedYes

Keywords

  • Antibiotic test
  • Membrane-filtration
  • Porous membrane

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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