Rapid detection of hemagglutination using restrictive microfluidic channels equipped with waveguide-mode sensors

Hiroki Ashiba, Makoto Fujimaki, Koichi Awazu, Mengying Fu, Yoshimichi Ohki, Torahiko Tanaka, Makoto Makishima

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Hemagglutination is utilized for various immunological assays, including blood typing and virus detection. Herein, we describe a method of rapid hemagglutination detection based on a microfluidic channel installed on an optical waveguide-mode sensor. Human blood samples mixed with hemagglutinating antibodies associated with different blood groups were injected into the microfluidic channel, and reflectance spectra of the samples were measured after stopping the flow. The agglutinated and nonagglutinated samples were distinguishable by the alterations in their reflectance spectra with time; the microfluidic channels worked as spatial restraints for agglutinated red blood cells. The demonstrated system allowed rapid hemagglutination detection within 1 min. The suitable height of the channels was also discussed.

    Original languageEnglish
    Article number027002
    JournalJapanese Journal of Applied Physics
    Volume55
    Issue number2
    DOIs
    Publication statusPublished - 2016 Feb 1

    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

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