Abstract
A rapid determination of aicotinic acid by using immobilized Lactobacillus arabinosus and a combined glass electrode is reported. L. arabinosus is immobilized in agar gel. The optimum agar concentration is from 2-2.5% ( w v) and the optimum bacterial concentration in the agar gel matrix is 10 mg wet cells ml-1. The relationship between the potential difference and the logarithm of the nicotinic acid concentration is linear over the range 5 X 10-8-5 X 10-6 g ml-1. The assay requires only 1 h; potentials are reproducible with an average relative error of 5%. The growth of L. arabinosus in agar gel matrix is observed in the medium containing nicotinic acid. Immobilized L. arabinosus is stable for 30 days.
| Original language | English |
|---|---|
| Pages (from-to) | 233-239 |
| Number of pages | 7 |
| Journal | Analytica Chimica Acta |
| Volume | 99 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1978 Aug 1 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
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Cite this
Matsunaga, T., Karube, I., & Suzuki, S. (1978). Rapid determination of nicotinic acid by immobilized lactobacillus arabinosus. Analytica Chimica Acta, 99(2), 233-239. https://doi.org/10.1016/S0003-2670(01)83562-5