Rapid determination of nicotinic acid by immobilized lactobacillus arabinosus

Tadashi Matsunaga, Isao Karube, Shuichi Suzuki

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A rapid determination of aicotinic acid by using immobilized Lactobacillus arabinosus and a combined glass electrode is reported. L. arabinosus is immobilized in agar gel. The optimum agar concentration is from 2-2.5% ( w v) and the optimum bacterial concentration in the agar gel matrix is 10 mg wet cells ml-1. The relationship between the potential difference and the logarithm of the nicotinic acid concentration is linear over the range 5 X 10-8-5 X 10-6 g ml-1. The assay requires only 1 h; potentials are reproducible with an average relative error of 5%. The growth of L. arabinosus in agar gel matrix is observed in the medium containing nicotinic acid. Immobilized L. arabinosus is stable for 30 days.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalAnalytica Chimica Acta
Volume99
Issue number2
DOIs
Publication statusPublished - 1978 Aug 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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