Rapid determination of nicotinic acid by immobilized lactobacillus arabinosus

Tadashi Matsunaga, Isao Karube, Shuichi Suzuki

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A rapid determination of aicotinic acid by using immobilized Lactobacillus arabinosus and a combined glass electrode is reported. L. arabinosus is immobilized in agar gel. The optimum agar concentration is from 2-2.5% ( w v) and the optimum bacterial concentration in the agar gel matrix is 10 mg wet cells ml-1. The relationship between the potential difference and the logarithm of the nicotinic acid concentration is linear over the range 5 X 10-8-5 X 10-6 g ml-1. The assay requires only 1 h; potentials are reproducible with an average relative error of 5%. The growth of L. arabinosus in agar gel matrix is observed in the medium containing nicotinic acid. Immobilized L. arabinosus is stable for 30 days.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalAnalytica Chimica Acta
Volume99
Issue number2
DOIs
Publication statusPublished - 1978 Aug 1
Externally publishedYes

Fingerprint

Niacin
Lactobacillus
agar
Agar
gel
Gels
acid
matrix
Glass
Assays
Electrodes
electrode
glass
assay
Acids
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Rapid determination of nicotinic acid by immobilized lactobacillus arabinosus. / Matsunaga, Tadashi; Karube, Isao; Suzuki, Shuichi.

In: Analytica Chimica Acta, Vol. 99, No. 2, 01.08.1978, p. 233-239.

Research output: Contribution to journalArticle

Matsunaga, Tadashi ; Karube, Isao ; Suzuki, Shuichi. / Rapid determination of nicotinic acid by immobilized lactobacillus arabinosus. In: Analytica Chimica Acta. 1978 ; Vol. 99, No. 2. pp. 233-239.
@article{ab136a5bbd294a8cb0507acb0fc204d4,
title = "Rapid determination of nicotinic acid by immobilized lactobacillus arabinosus",
abstract = "A rapid determination of aicotinic acid by using immobilized Lactobacillus arabinosus and a combined glass electrode is reported. L. arabinosus is immobilized in agar gel. The optimum agar concentration is from 2-2.5{\%} ( w v) and the optimum bacterial concentration in the agar gel matrix is 10 mg wet cells ml-1. The relationship between the potential difference and the logarithm of the nicotinic acid concentration is linear over the range 5 X 10-8-5 X 10-6 g ml-1. The assay requires only 1 h; potentials are reproducible with an average relative error of 5{\%}. The growth of L. arabinosus in agar gel matrix is observed in the medium containing nicotinic acid. Immobilized L. arabinosus is stable for 30 days.",
author = "Tadashi Matsunaga and Isao Karube and Shuichi Suzuki",
year = "1978",
month = "8",
day = "1",
doi = "10.1016/S0003-2670(01)83562-5",
language = "English",
volume = "99",
pages = "233--239",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Rapid determination of nicotinic acid by immobilized lactobacillus arabinosus

AU - Matsunaga, Tadashi

AU - Karube, Isao

AU - Suzuki, Shuichi

PY - 1978/8/1

Y1 - 1978/8/1

N2 - A rapid determination of aicotinic acid by using immobilized Lactobacillus arabinosus and a combined glass electrode is reported. L. arabinosus is immobilized in agar gel. The optimum agar concentration is from 2-2.5% ( w v) and the optimum bacterial concentration in the agar gel matrix is 10 mg wet cells ml-1. The relationship between the potential difference and the logarithm of the nicotinic acid concentration is linear over the range 5 X 10-8-5 X 10-6 g ml-1. The assay requires only 1 h; potentials are reproducible with an average relative error of 5%. The growth of L. arabinosus in agar gel matrix is observed in the medium containing nicotinic acid. Immobilized L. arabinosus is stable for 30 days.

AB - A rapid determination of aicotinic acid by using immobilized Lactobacillus arabinosus and a combined glass electrode is reported. L. arabinosus is immobilized in agar gel. The optimum agar concentration is from 2-2.5% ( w v) and the optimum bacterial concentration in the agar gel matrix is 10 mg wet cells ml-1. The relationship between the potential difference and the logarithm of the nicotinic acid concentration is linear over the range 5 X 10-8-5 X 10-6 g ml-1. The assay requires only 1 h; potentials are reproducible with an average relative error of 5%. The growth of L. arabinosus in agar gel matrix is observed in the medium containing nicotinic acid. Immobilized L. arabinosus is stable for 30 days.

UR - http://www.scopus.com/inward/record.url?scp=0004031796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0004031796&partnerID=8YFLogxK

U2 - 10.1016/S0003-2670(01)83562-5

DO - 10.1016/S0003-2670(01)83562-5

M3 - Article

VL - 99

SP - 233

EP - 239

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 2

ER -