Abstract
A rapid determination of aicotinic acid by using immobilized Lactobacillus arabinosus and a combined glass electrode is reported. L. arabinosus is immobilized in agar gel. The optimum agar concentration is from 2-2.5% ( w v) and the optimum bacterial concentration in the agar gel matrix is 10 mg wet cells ml-1. The relationship between the potential difference and the logarithm of the nicotinic acid concentration is linear over the range 5 X 10-8-5 X 10-6 g ml-1. The assay requires only 1 h; potentials are reproducible with an average relative error of 5%. The growth of L. arabinosus in agar gel matrix is observed in the medium containing nicotinic acid. Immobilized L. arabinosus is stable for 30 days.
Original language | English |
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Pages (from-to) | 233-239 |
Number of pages | 7 |
Journal | Analytica Chimica Acta |
Volume | 99 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1978 Aug 1 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry