Rapid DNA chemical ligation for amplification of RNA and DNA signal

Hiroshi Abe, Yuko Kondo, Hiroshi Jinmei, Naoko Abe, Kazuhiro Furukawa, Atsushi Uchiyama, Satoshi Tsuneda, Kyoko Aikawa, Isamu Matsumoto, Yoshihiro Ito

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Enzymatic ligation methods are useful in the diagnostic detection of DNA sequences. Here, we describe the investigation of nonenzymatic phosphorothioate-iodoacetyl DNA chemical ligation as a method for the detection and identification of RNA and DNA. The specificity of ligation on the DNA target is shown to allow the discrimination of a single point mutation with a drop in the ligation yield of up to 16.1-fold. Although enzymatic ligation has very low activity for RNA targets, this reaction is very efficient for RNA targets. The speed of the chemical ligation with an RNA target achieves a 70% yield in 5 s, which is equal to or better than that of ligase-enzyme-mediated ligation with a DNA target. The reaction also exhibits a significant level of signal amplification under thermal cycling in periods as short as 100-120 min, with the RNA or DNA target acting in a catalytic way to ligate multiple pairs of probes.

    Original languageEnglish
    Pages (from-to)327-333
    Number of pages7
    JournalBioconjugate Chemistry
    Volume19
    Issue number1
    DOIs
    Publication statusPublished - 2008 Jan

    Fingerprint

    RNA
    Ligation
    Amplification
    DNA
    DNA sequences
    Thermal cycling
    Ligases
    Enzymes
    Point Mutation
    Hot Temperature

    ASJC Scopus subject areas

    • Chemistry(all)
    • Organic Chemistry
    • Clinical Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry

    Cite this

    Abe, H., Kondo, Y., Jinmei, H., Abe, N., Furukawa, K., Uchiyama, A., ... Ito, Y. (2008). Rapid DNA chemical ligation for amplification of RNA and DNA signal. Bioconjugate Chemistry, 19(1), 327-333. https://doi.org/10.1021/bc700244s

    Rapid DNA chemical ligation for amplification of RNA and DNA signal. / Abe, Hiroshi; Kondo, Yuko; Jinmei, Hiroshi; Abe, Naoko; Furukawa, Kazuhiro; Uchiyama, Atsushi; Tsuneda, Satoshi; Aikawa, Kyoko; Matsumoto, Isamu; Ito, Yoshihiro.

    In: Bioconjugate Chemistry, Vol. 19, No. 1, 01.2008, p. 327-333.

    Research output: Contribution to journalArticle

    Abe, H, Kondo, Y, Jinmei, H, Abe, N, Furukawa, K, Uchiyama, A, Tsuneda, S, Aikawa, K, Matsumoto, I & Ito, Y 2008, 'Rapid DNA chemical ligation for amplification of RNA and DNA signal', Bioconjugate Chemistry, vol. 19, no. 1, pp. 327-333. https://doi.org/10.1021/bc700244s
    Abe H, Kondo Y, Jinmei H, Abe N, Furukawa K, Uchiyama A et al. Rapid DNA chemical ligation for amplification of RNA and DNA signal. Bioconjugate Chemistry. 2008 Jan;19(1):327-333. https://doi.org/10.1021/bc700244s
    Abe, Hiroshi ; Kondo, Yuko ; Jinmei, Hiroshi ; Abe, Naoko ; Furukawa, Kazuhiro ; Uchiyama, Atsushi ; Tsuneda, Satoshi ; Aikawa, Kyoko ; Matsumoto, Isamu ; Ito, Yoshihiro. / Rapid DNA chemical ligation for amplification of RNA and DNA signal. In: Bioconjugate Chemistry. 2008 ; Vol. 19, No. 1. pp. 327-333.
    @article{c2b77882bc074659a157c0085389754f,
    title = "Rapid DNA chemical ligation for amplification of RNA and DNA signal",
    abstract = "Enzymatic ligation methods are useful in the diagnostic detection of DNA sequences. Here, we describe the investigation of nonenzymatic phosphorothioate-iodoacetyl DNA chemical ligation as a method for the detection and identification of RNA and DNA. The specificity of ligation on the DNA target is shown to allow the discrimination of a single point mutation with a drop in the ligation yield of up to 16.1-fold. Although enzymatic ligation has very low activity for RNA targets, this reaction is very efficient for RNA targets. The speed of the chemical ligation with an RNA target achieves a 70{\%} yield in 5 s, which is equal to or better than that of ligase-enzyme-mediated ligation with a DNA target. The reaction also exhibits a significant level of signal amplification under thermal cycling in periods as short as 100-120 min, with the RNA or DNA target acting in a catalytic way to ligate multiple pairs of probes.",
    author = "Hiroshi Abe and Yuko Kondo and Hiroshi Jinmei and Naoko Abe and Kazuhiro Furukawa and Atsushi Uchiyama and Satoshi Tsuneda and Kyoko Aikawa and Isamu Matsumoto and Yoshihiro Ito",
    year = "2008",
    month = "1",
    doi = "10.1021/bc700244s",
    language = "English",
    volume = "19",
    pages = "327--333",
    journal = "Bioconjugate Chemistry",
    issn = "1043-1802",
    publisher = "American Chemical Society",
    number = "1",

    }

    TY - JOUR

    T1 - Rapid DNA chemical ligation for amplification of RNA and DNA signal

    AU - Abe, Hiroshi

    AU - Kondo, Yuko

    AU - Jinmei, Hiroshi

    AU - Abe, Naoko

    AU - Furukawa, Kazuhiro

    AU - Uchiyama, Atsushi

    AU - Tsuneda, Satoshi

    AU - Aikawa, Kyoko

    AU - Matsumoto, Isamu

    AU - Ito, Yoshihiro

    PY - 2008/1

    Y1 - 2008/1

    N2 - Enzymatic ligation methods are useful in the diagnostic detection of DNA sequences. Here, we describe the investigation of nonenzymatic phosphorothioate-iodoacetyl DNA chemical ligation as a method for the detection and identification of RNA and DNA. The specificity of ligation on the DNA target is shown to allow the discrimination of a single point mutation with a drop in the ligation yield of up to 16.1-fold. Although enzymatic ligation has very low activity for RNA targets, this reaction is very efficient for RNA targets. The speed of the chemical ligation with an RNA target achieves a 70% yield in 5 s, which is equal to or better than that of ligase-enzyme-mediated ligation with a DNA target. The reaction also exhibits a significant level of signal amplification under thermal cycling in periods as short as 100-120 min, with the RNA or DNA target acting in a catalytic way to ligate multiple pairs of probes.

    AB - Enzymatic ligation methods are useful in the diagnostic detection of DNA sequences. Here, we describe the investigation of nonenzymatic phosphorothioate-iodoacetyl DNA chemical ligation as a method for the detection and identification of RNA and DNA. The specificity of ligation on the DNA target is shown to allow the discrimination of a single point mutation with a drop in the ligation yield of up to 16.1-fold. Although enzymatic ligation has very low activity for RNA targets, this reaction is very efficient for RNA targets. The speed of the chemical ligation with an RNA target achieves a 70% yield in 5 s, which is equal to or better than that of ligase-enzyme-mediated ligation with a DNA target. The reaction also exhibits a significant level of signal amplification under thermal cycling in periods as short as 100-120 min, with the RNA or DNA target acting in a catalytic way to ligate multiple pairs of probes.

    UR - http://www.scopus.com/inward/record.url?scp=38949192864&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=38949192864&partnerID=8YFLogxK

    U2 - 10.1021/bc700244s

    DO - 10.1021/bc700244s

    M3 - Article

    C2 - 17990846

    AN - SCOPUS:38949192864

    VL - 19

    SP - 327

    EP - 333

    JO - Bioconjugate Chemistry

    JF - Bioconjugate Chemistry

    SN - 1043-1802

    IS - 1

    ER -