Rapid hepatic fate specification of adipose-derived stem cells and their therapeutic potential for liver failure

Agnieszka Banas, Takumi Teratani, Yusuke Yamamoto, Makoto Tokuhara, Fumitaka Takeshita, Mitsuhiko Osaki, Takashi Kato, Hitoshi Okochi, Takahiro Ochiya

    Research output: Contribution to journalArticle

    193 Citations (Scopus)

    Abstract

    Background and Aim: Multipotential mesenchymal stem cells (MSC), present in many organs and tissues, represent an attractive tool for the establishment of a successful stem cell-based therapy in the field of regeneration medicine. Adipose tissue mesenchymal stem cells (AT-MSC), known as adipose-derived stem cells (ASC) are especially attractive in the context of future clinical applications because of their high accessibility and minimal invasiveness during the procedure to obtain them. The goal of the present study was to induce human ASC into functional hepatocytes in vitro within a very short period of time and to check their therapeutic potential in vivo. Methods: In vitro generated ASC-derived hepatocytes were checked for hepatocyte-specific markers and functions. Afterwards, they were transplanted into nude mice with liver injury. Twenty-four hours after transplantation, biochemical parameters were evaluated in blood serum. Results: We have shown here that ASC can be differentiated into hepatocytes within 13 days and can reach the functional properties of primary human hepatocytes. After transplantation into mice with acute liver failure, ASC-derived hepatocytes can restore such liver functions as ammonia and purine metabolism. Markers of liver injury, alanine aminotransferase, aspartate aminotransferase, as well as ammonia, were decreased after ASC-derived hepatocyte transplantation. Conclusions: Our data highlight the properties of ASC as having a special affinity for hepatocyte differentiation in vitro and liver regeneration in vivo. Thus, ASC may be a superior choice for the establishment of a therapy for injured liver.

    Original languageEnglish
    Pages (from-to)70-77
    Number of pages8
    JournalJournal of Gastroenterology and Hepatology (Australia)
    Volume24
    Issue number1
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    Liver Failure
    Stem Cells
    Hepatocytes
    Liver
    Therapeutics
    Transplantation
    Mesenchymal Stromal Cells
    Ammonia
    Liver Regeneration
    Acute Liver Failure
    Wounds and Injuries
    Cell- and Tissue-Based Therapy
    Aspartate Aminotransferases
    Alanine Transaminase
    Nude Mice
    Adipose Tissue
    Regeneration
    Medicine
    Serum

    Keywords

    • Adipose
    • Differentiation
    • Hepatocyte
    • Liver regeneration
    • Mesenchymal stem cell

    ASJC Scopus subject areas

    • Gastroenterology
    • Hepatology

    Cite this

    Rapid hepatic fate specification of adipose-derived stem cells and their therapeutic potential for liver failure. / Banas, Agnieszka; Teratani, Takumi; Yamamoto, Yusuke; Tokuhara, Makoto; Takeshita, Fumitaka; Osaki, Mitsuhiko; Kato, Takashi; Okochi, Hitoshi; Ochiya, Takahiro.

    In: Journal of Gastroenterology and Hepatology (Australia), Vol. 24, No. 1, 2009, p. 70-77.

    Research output: Contribution to journalArticle

    Banas, Agnieszka ; Teratani, Takumi ; Yamamoto, Yusuke ; Tokuhara, Makoto ; Takeshita, Fumitaka ; Osaki, Mitsuhiko ; Kato, Takashi ; Okochi, Hitoshi ; Ochiya, Takahiro. / Rapid hepatic fate specification of adipose-derived stem cells and their therapeutic potential for liver failure. In: Journal of Gastroenterology and Hepatology (Australia). 2009 ; Vol. 24, No. 1. pp. 70-77.
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