Rapid imaging and detection of circulating tumor cells using a wide-field fluorescence imaging system

Tomoko Yoshino, Kaori Takai, Ryo Negishi, Tatsuya Saeki, Hisashige Kanbara, Yoshihito Kikuhara, Tadashi Matsunaga, Tsuyoshi Tanaka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) provide potentially accessible in vivo sources of metastatic cancer cells. As such, considerable focus has been placed on analyzing the genetics of single-CTCs. Prior to these analyses, however, CTCs must first be detected within the blood samples of cancer patients. Current methods for detection of CTCs by fluorescence microscopy require the analysis of hundreds of images per blood sample, making this a time-consuming process that creates a bottleneck in CTC analysis. In this study, we therefore developed a wide-field fluorescence imaging system for rapid CTC detection. For these analyses, CTCs were first isolated using the microcavity array (MCA), a micro-sized filter for CTC recovery that separates cells based on differences in cell size and deformability. Notably, the proposed imaging system enabled rapid (∼10 s) visualization of all stained cells within the 6 mm × 6 mm MCA field via one-shot imaging. Furthermore, the morphology of the cells in the resulting images accurately reflected that observed by conventional microscopy. In total, isolation and detection of CTCs using the MCA combined with our novel wide-field fluorescence imaging system was achieved within 1 h. Thus, our proposed system will provide rapid CTC detection system.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalAnalytica Chimica Acta
Volume969
DOIs
Publication statusPublished - 2017 May 29
Externally publishedYes

Keywords

  • Circulating tumor cells
  • CMOS sensor
  • Fluorescence
  • Microcavity array
  • One shot imaging
  • Wide-field imaging

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Biochemistry
  • Spectroscopy

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  • Cite this

    Yoshino, T., Takai, K., Negishi, R., Saeki, T., Kanbara, H., Kikuhara, Y., Matsunaga, T., & Tanaka, T. (2017). Rapid imaging and detection of circulating tumor cells using a wide-field fluorescence imaging system. Analytica Chimica Acta, 969, 1-7. https://doi.org/10.1016/j.aca.2017.03.030