Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

N. Noda, H. Ikuta, Y. Ebie, A. Hirata, Satoshi Tsuneda, M. Matsumura, T. Sumino, Y. Inamori

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed bacterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO 14298) and sixteen against Nitrobacter winogradskyi (IFO 14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

    Original languageEnglish
    Pages (from-to)301-308
    Number of pages8
    JournalWater Science and Technology
    Volume41
    Issue number4-5
    Publication statusPublished - 2000

    Fingerprint

    nitrifying bacterium
    Monoclonal antibodies
    Biofilms
    antibody
    biofilm
    Bacteria
    Antibodies
    Leachate treatment
    Land fill
    Polyethylene glycols
    Gels
    bacterium
    detection
    in situ
    method
    activated sludge
    gel
    habitat

    Keywords

    • Biofilm
    • In situ fluorescent antibody technique
    • Monoclonal antibody
    • Nitrifying bacteria
    • Quantification

    ASJC Scopus subject areas

    • Water Science and Technology

    Cite this

    Noda, N., Ikuta, H., Ebie, Y., Hirata, A., Tsuneda, S., Matsumura, M., ... Inamori, Y. (2000). Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method. Water Science and Technology, 41(4-5), 301-308.

    Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method. / Noda, N.; Ikuta, H.; Ebie, Y.; Hirata, A.; Tsuneda, Satoshi; Matsumura, M.; Sumino, T.; Inamori, Y.

    In: Water Science and Technology, Vol. 41, No. 4-5, 2000, p. 301-308.

    Research output: Contribution to journalArticle

    Noda, N, Ikuta, H, Ebie, Y, Hirata, A, Tsuneda, S, Matsumura, M, Sumino, T & Inamori, Y 2000, 'Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method', Water Science and Technology, vol. 41, no. 4-5, pp. 301-308.
    Noda, N. ; Ikuta, H. ; Ebie, Y. ; Hirata, A. ; Tsuneda, Satoshi ; Matsumura, M. ; Sumino, T. ; Inamori, Y. / Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method. In: Water Science and Technology. 2000 ; Vol. 41, No. 4-5. pp. 301-308.
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