Rapid quantitative method for the detection of phenylalanine and tyrosine in human plasma using pillar array columns and gradient elution

Yanting Song, Katsuya Takatsuki, Tetsushi Sekiguchi, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This study reports a fast and quantitative determination method for phenylalanine (Phe) and tyrosine (Tyr) in human plasma using on-chip pressure-driven liquid chromatography. A pillar array column with low-dispersion turns and a gradient elution system was used. The separation of fluorescent derivatives of Phe, Tyr, and other hydrophobic amino acids was successfully performed within 140 s. Under the optimized conditions, Phe and Tyr in human plasma were quantified. The developed method is promising for rapid diagnosis in the clinical field.

Original languageEnglish
Pages (from-to)1731-1735
Number of pages5
JournalAmino Acids
Volume48
Issue number7
DOIs
Publication statusPublished - 2016 Jul 1

Keywords

  • 4-Fluoro-7-nitro-2,1,3-benzoxadiazole
  • Amino acids
  • Fluorescence
  • Microfluidics
  • Quantitative determination

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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