Rapid quantitative method for the detection of phenylalanine and tyrosine in human plasma using pillar array columns and gradient elution

Yanting Song, Katsuya Takatsuki, Tetsushi Sekiguchi, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study reports a fast and quantitative determination method for phenylalanine (Phe) and tyrosine (Tyr) in human plasma using on-chip pressure-driven liquid chromatography. A pillar array column with low-dispersion turns and a gradient elution system was used. The separation of fluorescent derivatives of Phe, Tyr, and other hydrophobic amino acids was successfully performed within 140 s. Under the optimized conditions, Phe and Tyr in human plasma were quantified. The developed method is promising for rapid diagnosis in the clinical field.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalAmino Acids
DOIs
Publication statusAccepted/In press - 2016 May 21

Fingerprint

Plasma (human)
Phenylalanine
Tyrosine
Liquid chromatography
Liquid Chromatography
Derivatives
Amino Acids
Pressure

Keywords

  • 4-Fluoro-7-nitro-2,1,3-benzoxadiazole
  • Amino acids
  • Fluorescence
  • Microfluidics
  • Quantitative determination

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Rapid quantitative method for the detection of phenylalanine and tyrosine in human plasma using pillar array columns and gradient elution. / Song, Yanting; Takatsuki, Katsuya; Sekiguchi, Tetsushi; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto.

In: Amino Acids, 21.05.2016, p. 1-5.

Research output: Contribution to journalArticle

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