Rapid recovery of bacterial cells from a stable dispersion by heterocoagulation to a fibrous collector

Hiroshi Hayashi, Tomoya Nihei, Motomi Ono, Satoshi Tsuneda, Akira Hirata, Hiroshi Sasaki

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Rapid recovery of five heterotrophs, Escherichia coli, Pseudomonas putida, Pseudomonas aeruginosa, Alcaligenes sp., and Alcaligenes faecalis, was investigated at various pH and ionic concentrations and the results were compared with data of the electrophoretic mobility of cells and substrata. Cell recovery was performed by a column bed packed with fibrous ferro-nickel slag, which was utilized as the collector media of bacterial cells. In the case of P. putida, which showed negative electrophoretic mobility, a low cell recovery was obtained at pH 7.0, whereas the cell collection ratio gradually increased as the pH decreased. The collection of A. faecalis improved as the ionic concentration increased. Considering the adhesion of bacterial cells to fibrous slag as the heterocoagulation between cell and substrata, these findings were explained by the reduction of electrostatic repulsive interaction between cell and substrata, leading to an increase of cell recovery. On the other hand, separation of P. aeruginosa, whose electrophoretic mobility was almost zero under all experimental conditions, hardly depended on the chemical properties of the suspension. Cell collection behavior was qualitatively in good agreement with electrokinetic properties of cells and slag, suggesting that cell recovery based on surface characteristics is a promising method, especially for stable bacterial dispersion.

    Original languageEnglish
    Pages (from-to)109-115
    Number of pages7
    JournalJournal of Colloid and Interface Science
    Volume243
    Issue number1
    DOIs
    Publication statusPublished - 2001 Nov 1

    Fingerprint

    accumulators
    Electrophoretic mobility
    recovery
    Recovery
    Slags
    cells
    slags
    Packed beds
    Coulomb interactions
    Nickel
    pseudomonas
    Chemical properties
    Escherichia coli
    Suspensions
    Adhesion
    heterotrophs
    Cells
    electrokinetics
    Escherichia
    chemical properties

    Keywords

    • Cell collection capacity
    • Electrophoretic mobility
    • Electrostatic interaction
    • Fibrous slag
    • Heterocoagulation
    • Recovery of bacteria

    ASJC Scopus subject areas

    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry
    • Surfaces and Interfaces

    Cite this

    Rapid recovery of bacterial cells from a stable dispersion by heterocoagulation to a fibrous collector. / Hayashi, Hiroshi; Nihei, Tomoya; Ono, Motomi; Tsuneda, Satoshi; Hirata, Akira; Sasaki, Hiroshi.

    In: Journal of Colloid and Interface Science, Vol. 243, No. 1, 01.11.2001, p. 109-115.

    Research output: Contribution to journalArticle

    Hayashi, Hiroshi ; Nihei, Tomoya ; Ono, Motomi ; Tsuneda, Satoshi ; Hirata, Akira ; Sasaki, Hiroshi. / Rapid recovery of bacterial cells from a stable dispersion by heterocoagulation to a fibrous collector. In: Journal of Colloid and Interface Science. 2001 ; Vol. 243, No. 1. pp. 109-115.
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    abstract = "Rapid recovery of five heterotrophs, Escherichia coli, Pseudomonas putida, Pseudomonas aeruginosa, Alcaligenes sp., and Alcaligenes faecalis, was investigated at various pH and ionic concentrations and the results were compared with data of the electrophoretic mobility of cells and substrata. Cell recovery was performed by a column bed packed with fibrous ferro-nickel slag, which was utilized as the collector media of bacterial cells. In the case of P. putida, which showed negative electrophoretic mobility, a low cell recovery was obtained at pH 7.0, whereas the cell collection ratio gradually increased as the pH decreased. The collection of A. faecalis improved as the ionic concentration increased. Considering the adhesion of bacterial cells to fibrous slag as the heterocoagulation between cell and substrata, these findings were explained by the reduction of electrostatic repulsive interaction between cell and substrata, leading to an increase of cell recovery. On the other hand, separation of P. aeruginosa, whose electrophoretic mobility was almost zero under all experimental conditions, hardly depended on the chemical properties of the suspension. Cell collection behavior was qualitatively in good agreement with electrokinetic properties of cells and slag, suggesting that cell recovery based on surface characteristics is a promising method, especially for stable bacterial dispersion.",
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