Rare occurrence of ras and p53 gene mutations in mouse stomach tumors induced by N-methyl-N-nitrosourea

Chie Furihata, Masae Tatematsu, Masae Saito, Shizuka Ishida, Hayao Nakanishi, Ken Ichi Inada, Hajime Tei, Masahira Hattori, Takashi Ito, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The incidence of point mutations of H-, K- and N-ras and p53 oncogenes in male BALB/c mouse stomach tumors induced with N-methyl-N-nitrosourea (MNU) was examined by direct sequencing and PCR single-strand conformation polymorphism (PCR-SSCP). A mutation of GGT to AGT at K-ras codon 12 was found by SSCP in one adenocarcinoma from a total of 19 specimens including 5 adenocarcinomas, 9 adenomatous hyperplastic regions, 1 squamous cell carcinoma and 4 normal-like stomach regions from 4 mice. No mutations were detected by direct sequencing of H-, K- and N-ras oncogenes at exons 1 (codons 12 and 13) and 2 (codon 61) in a total of 26 specimens comprising 10 adenocarcinomas, 10 adenomatous hyperplastic regions, 2 squamous cell carcinomas and 4 normal-like stomach regions from 6 mice. No mutations were detected by direct sequencing of p53 oncogene at exons 5, 6, 7 and 8 in a total of 30 specimens including 13 adenocarcinomas, 8 adenomatous hyperplastic regions, 2 squamous cell carcinomas, 1 papilloma and 6 normal-like stomach regions from 7 mice. These results suggest that ras and p53 oncogenes do not play a role in mouse stomach carcinogenesis induced by MNU.

Original languageEnglish
Pages (from-to)363-368
Number of pages6
JournalJapanese Journal of Cancer Research
Volume88
Issue number4
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Methylnitrosourea
ras Genes
p53 Genes
Stomach
Adenocarcinoma
Mutation
Codon
Squamous Cell Carcinoma
Neoplasms
Exons
Single-Stranded Conformational Polymorphism
Papilloma
Oncogenes
Point Mutation
Carcinogenesis
Polymerase Chain Reaction
Incidence

Keywords

  • Mouse
  • N-methyl-N-nitrosourea
  • p53
  • ras
  • Stomach tumor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Furihata, C., Tatematsu, M., Saito, M., Ishida, S., Nakanishi, H., Inada, K. I., ... Sakaki, Y. (1997). Rare occurrence of ras and p53 gene mutations in mouse stomach tumors induced by N-methyl-N-nitrosourea. Japanese Journal of Cancer Research, 88(4), 363-368.

Rare occurrence of ras and p53 gene mutations in mouse stomach tumors induced by N-methyl-N-nitrosourea. / Furihata, Chie; Tatematsu, Masae; Saito, Masae; Ishida, Shizuka; Nakanishi, Hayao; Inada, Ken Ichi; Tei, Hajime; Hattori, Masahira; Ito, Takashi; Sakaki, Yoshiyuki.

In: Japanese Journal of Cancer Research, Vol. 88, No. 4, 1997, p. 363-368.

Research output: Contribution to journalArticle

Furihata, C, Tatematsu, M, Saito, M, Ishida, S, Nakanishi, H, Inada, KI, Tei, H, Hattori, M, Ito, T & Sakaki, Y 1997, 'Rare occurrence of ras and p53 gene mutations in mouse stomach tumors induced by N-methyl-N-nitrosourea', Japanese Journal of Cancer Research, vol. 88, no. 4, pp. 363-368.
Furihata C, Tatematsu M, Saito M, Ishida S, Nakanishi H, Inada KI et al. Rare occurrence of ras and p53 gene mutations in mouse stomach tumors induced by N-methyl-N-nitrosourea. Japanese Journal of Cancer Research. 1997;88(4):363-368.
Furihata, Chie ; Tatematsu, Masae ; Saito, Masae ; Ishida, Shizuka ; Nakanishi, Hayao ; Inada, Ken Ichi ; Tei, Hajime ; Hattori, Masahira ; Ito, Takashi ; Sakaki, Yoshiyuki. / Rare occurrence of ras and p53 gene mutations in mouse stomach tumors induced by N-methyl-N-nitrosourea. In: Japanese Journal of Cancer Research. 1997 ; Vol. 88, No. 4. pp. 363-368.
@article{f37673c825344604a98b4ecf909d0ed4,
title = "Rare occurrence of ras and p53 gene mutations in mouse stomach tumors induced by N-methyl-N-nitrosourea",
abstract = "The incidence of point mutations of H-, K- and N-ras and p53 oncogenes in male BALB/c mouse stomach tumors induced with N-methyl-N-nitrosourea (MNU) was examined by direct sequencing and PCR single-strand conformation polymorphism (PCR-SSCP). A mutation of GGT to AGT at K-ras codon 12 was found by SSCP in one adenocarcinoma from a total of 19 specimens including 5 adenocarcinomas, 9 adenomatous hyperplastic regions, 1 squamous cell carcinoma and 4 normal-like stomach regions from 4 mice. No mutations were detected by direct sequencing of H-, K- and N-ras oncogenes at exons 1 (codons 12 and 13) and 2 (codon 61) in a total of 26 specimens comprising 10 adenocarcinomas, 10 adenomatous hyperplastic regions, 2 squamous cell carcinomas and 4 normal-like stomach regions from 6 mice. No mutations were detected by direct sequencing of p53 oncogene at exons 5, 6, 7 and 8 in a total of 30 specimens including 13 adenocarcinomas, 8 adenomatous hyperplastic regions, 2 squamous cell carcinomas, 1 papilloma and 6 normal-like stomach regions from 7 mice. These results suggest that ras and p53 oncogenes do not play a role in mouse stomach carcinogenesis induced by MNU.",
keywords = "Mouse, N-methyl-N-nitrosourea, p53, ras, Stomach tumor",
author = "Chie Furihata and Masae Tatematsu and Masae Saito and Shizuka Ishida and Hayao Nakanishi and Inada, {Ken Ichi} and Hajime Tei and Masahira Hattori and Takashi Ito and Yoshiyuki Sakaki",
year = "1997",
language = "English",
volume = "88",
pages = "363--368",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Rare occurrence of ras and p53 gene mutations in mouse stomach tumors induced by N-methyl-N-nitrosourea

AU - Furihata, Chie

AU - Tatematsu, Masae

AU - Saito, Masae

AU - Ishida, Shizuka

AU - Nakanishi, Hayao

AU - Inada, Ken Ichi

AU - Tei, Hajime

AU - Hattori, Masahira

AU - Ito, Takashi

AU - Sakaki, Yoshiyuki

PY - 1997

Y1 - 1997

N2 - The incidence of point mutations of H-, K- and N-ras and p53 oncogenes in male BALB/c mouse stomach tumors induced with N-methyl-N-nitrosourea (MNU) was examined by direct sequencing and PCR single-strand conformation polymorphism (PCR-SSCP). A mutation of GGT to AGT at K-ras codon 12 was found by SSCP in one adenocarcinoma from a total of 19 specimens including 5 adenocarcinomas, 9 adenomatous hyperplastic regions, 1 squamous cell carcinoma and 4 normal-like stomach regions from 4 mice. No mutations were detected by direct sequencing of H-, K- and N-ras oncogenes at exons 1 (codons 12 and 13) and 2 (codon 61) in a total of 26 specimens comprising 10 adenocarcinomas, 10 adenomatous hyperplastic regions, 2 squamous cell carcinomas and 4 normal-like stomach regions from 6 mice. No mutations were detected by direct sequencing of p53 oncogene at exons 5, 6, 7 and 8 in a total of 30 specimens including 13 adenocarcinomas, 8 adenomatous hyperplastic regions, 2 squamous cell carcinomas, 1 papilloma and 6 normal-like stomach regions from 7 mice. These results suggest that ras and p53 oncogenes do not play a role in mouse stomach carcinogenesis induced by MNU.

AB - The incidence of point mutations of H-, K- and N-ras and p53 oncogenes in male BALB/c mouse stomach tumors induced with N-methyl-N-nitrosourea (MNU) was examined by direct sequencing and PCR single-strand conformation polymorphism (PCR-SSCP). A mutation of GGT to AGT at K-ras codon 12 was found by SSCP in one adenocarcinoma from a total of 19 specimens including 5 adenocarcinomas, 9 adenomatous hyperplastic regions, 1 squamous cell carcinoma and 4 normal-like stomach regions from 4 mice. No mutations were detected by direct sequencing of H-, K- and N-ras oncogenes at exons 1 (codons 12 and 13) and 2 (codon 61) in a total of 26 specimens comprising 10 adenocarcinomas, 10 adenomatous hyperplastic regions, 2 squamous cell carcinomas and 4 normal-like stomach regions from 6 mice. No mutations were detected by direct sequencing of p53 oncogene at exons 5, 6, 7 and 8 in a total of 30 specimens including 13 adenocarcinomas, 8 adenomatous hyperplastic regions, 2 squamous cell carcinomas, 1 papilloma and 6 normal-like stomach regions from 7 mice. These results suggest that ras and p53 oncogenes do not play a role in mouse stomach carcinogenesis induced by MNU.

KW - Mouse

KW - N-methyl-N-nitrosourea

KW - p53

KW - ras

KW - Stomach tumor

UR - http://www.scopus.com/inward/record.url?scp=0030951239&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030951239&partnerID=8YFLogxK

M3 - Article

C2 - 9197527

AN - SCOPUS:0030951239

VL - 88

SP - 363

EP - 368

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 4

ER -