Real-time detection of DNA hybridization on microarray using a CCD-based imaging system equipped with a rotated microlens array disk

Takeyuki Mogi, Keiichi Hatakeyama, Tomoyuki Taguchi, Hitoshi Wake, Takeo Tanaami, Masahito Hosokawa, Tsuyoshi Tanaka, Tadashi Matsunaga

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This work describes a novel charge-coupled device (CCD)-based imaging system (MB Biochip Reader™) for real-time detection of DNA hybridization to DNA microarrays. The MB Biochip Reader™ consisted of a laser light source (532. nm), a microlens array for generation of a multi-beam laser, and a CCD for 2-D signal imaging. The MB Biochip Reader™ with a rotated microlens array, allowed large-field imaging (6.2. mm×7.6. mm with 6.45μm resolution) with fast time-resolution at 0.2. s without speckle noise. Furthermore, real-time detection of DNA hybridization, which is sufficient to obtain accurate data from tens of thousands of array element per field, was successfully performed without the need for laser scanning. The performance of the MB Biochip Reader™ for DNA microarray imaging was similar to the commercially available photomultiplier tube (PMT)-based microarray scanner, ScanArray Lite. The system potentially could be applied toward real-time analysis in many other fluorescent techniques in addition to real-time DNA microarray analysis.

Original languageEnglish
Pages (from-to)1942-1946
Number of pages5
JournalBiosensors and Bioelectronics
Volume26
Issue number5
DOIs
Publication statusPublished - 2011 Jan 15
Externally publishedYes

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Keywords

  • Charge-coupled device (CCD)
  • DNA microarray
  • Microlens array
  • Molecular beacon
  • Real-time detection

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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