Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

Tatsuya Munaka, Hirohisa Abe, Masaki Kanai, Takashi Sakamoto, Hiroaki Nakanishi, Shuichi Shoji, Shinya Kimura, Taira Maekawa, Akira Murakami

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis.

    Original languageEnglish
    Pages (from-to)512-514
    Number of pages3
    JournalAnalyst
    Volume132
    Issue number6
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Mitogens
    Antibodies
    secretion
    antibody
    Anisotropy
    anisotropy
    B-Lymphocytes
    fluorescence
    Fluorescence
    Cells
    Fluorescence Polarization
    Monitoring
    monitoring
    analysis

    ASJC Scopus subject areas

    • Analytical Chemistry

    Cite this

    Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen. / Munaka, Tatsuya; Abe, Hirohisa; Kanai, Masaki; Sakamoto, Takashi; Nakanishi, Hiroaki; Shoji, Shuichi; Kimura, Shinya; Maekawa, Taira; Murakami, Akira.

    In: Analyst, Vol. 132, No. 6, 2007, p. 512-514.

    Research output: Contribution to journalArticle

    Munaka, T, Abe, H, Kanai, M, Sakamoto, T, Nakanishi, H, Shoji, S, Kimura, S, Maekawa, T & Murakami, A 2007, 'Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen', Analyst, vol. 132, no. 6, pp. 512-514. https://doi.org/10.1039/b701049d
    Munaka, Tatsuya ; Abe, Hirohisa ; Kanai, Masaki ; Sakamoto, Takashi ; Nakanishi, Hiroaki ; Shoji, Shuichi ; Kimura, Shinya ; Maekawa, Taira ; Murakami, Akira. / Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen. In: Analyst. 2007 ; Vol. 132, No. 6. pp. 512-514.
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