Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

Tatsuya Munaka, Hirohisa Abe, Masaki Kanai, Takashi Sakamoto, Hiroaki Nakanishi, Shuichi Shoji, Shinya Kimura, Taira Maekawa, Akira Murakami

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis.

Original languageEnglish
Pages (from-to)512-514
Number of pages3
JournalAnalyst
Volume132
Issue number6
DOIs
Publication statusPublished - 2007 Jan 1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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  • Cite this

    Munaka, T., Abe, H., Kanai, M., Sakamoto, T., Nakanishi, H., Shoji, S., Kimura, S., Maekawa, T., & Murakami, A. (2007). Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen. Analyst, 132(6), 512-514. https://doi.org/10.1039/b701049d