Real-time monitoring of voltage shift based on enzymatically released pyrophosphate using phenylboronic acid-immobilized gate field-effect transistor

Hirokazu Nishida, Kiyofumi Takahashi, Yuki Tabuse, Hideki Kambara, Toshiya Sakata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pyrophosphate (PPi) is ubiquitous in living cells and is often produced by enzymatic reactions, e.g., DNA synthesis by DNA polymerase. We have developed a novel detection system for the voltage shift associated with the change in PPi concentration resulting from an enzymatic reaction using a phenylboronic acid (PBA)-coated gate field-effect transistor (FET), since PBA coating is effective for detecting ion accumulation associated with PPi production from enzymatic reactions. To detect enzymatic reactions more efficiently, we employed the enzyme-electrode conjugation method using specific peptide sequences, which are spontaneously tethered to a gold substrate. The combination of the enzyme-electrode conjugation method with the charge detection using the PBA-coated FET enables the effective detection of enzymatic reactions.

Original languageEnglish
Article number04EL04
JournalJapanese Journal of Applied Physics
Volume53
Issue number4 SPEC. ISSUE
DOIs
Publication statusPublished - 2014

Fingerprint

Gates (transistor)
Enzyme electrodes
field effect transistors
acids
Acids
Monitoring
shift
Electric potential
electric potential
DNA
conjugation
enzymes
Field effect transistors
deoxyribonucleic acid
Peptides
Gold
Cells
electrodes
Coatings
Ions

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Real-time monitoring of voltage shift based on enzymatically released pyrophosphate using phenylboronic acid-immobilized gate field-effect transistor. / Nishida, Hirokazu; Takahashi, Kiyofumi; Tabuse, Yuki; Kambara, Hideki; Sakata, Toshiya.

In: Japanese Journal of Applied Physics, Vol. 53, No. 4 SPEC. ISSUE, 04EL04, 2014.

Research output: Contribution to journalArticle

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