Real time PCR based on fluorescent quenching of mercaptoacetic acid-modified cdte quantum dots for ultrasensitive specific detection of nucleic acids

Daxiang Cui, Qing Li, Peng Huang, Kan Wang, Yifei Kong, Hong Zhang, Xiaogang You, Rong He, Hua Song, Jingping Wang, Chenchen Bao, Toru Asahi, Feng Gao, Tetsuya Osaka

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A ultra-sensitive, highly specific, real-time polymerase chain reaction system based on mercaptoacetic acid-modified CdTe nanocrystals(mQDs) is reported. With the addition of 3 nm mQDs into the PCR reagent, the photoluminescent(PL) intensities of mQDs decreased gradually as the DNA templates and PCR cycles increased, in an approximate negative linear relation to the DNA concentration logarithm or cycles, the PL peaks exhibited red-shifts synchronously. Mg2+ ions decreased the PL intensity of mQDs in a dose-dependent means, and Taq DNA polymerase enhanced the PL intensity of mQDs in a dose-dependent means. Real-time PCR based on mQDs showed an increased sensitivity at least 103 fold higher than that based on SYBR Green I. The specificity of PCR was enhanced in the PCR reagent with less than 1.33mg/mL mQDs. The potential mechanism is also discussed. This novel PCR system based on mQDs has great potential in applications such as ultra-sensitive specific DNA or RNA detection, dynamic molecular imaging, and photoelectric biosensors.

Original languageEnglish
Pages (from-to)45-55
Number of pages11
JournalNano Biomedicine and Engineering
Volume2
Issue number1
Publication statusPublished - 2010

Fingerprint

Nucleic acids
Semiconductor quantum dots
Quenching
DNA
Acids
Molecular imaging
Polymerase chain reaction
RNA
Biosensors
Nanocrystals
Ions

Keywords

  • Mercaptoacetic acid-modified cdte quantum dot
  • Photoluminescence
  • Polymerase chain reaction
  • Sensitivity
  • Specificity

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Real time PCR based on fluorescent quenching of mercaptoacetic acid-modified cdte quantum dots for ultrasensitive specific detection of nucleic acids. / Cui, Daxiang; Li, Qing; Huang, Peng; Wang, Kan; Kong, Yifei; Zhang, Hong; You, Xiaogang; He, Rong; Song, Hua; Wang, Jingping; Bao, Chenchen; Asahi, Toru; Gao, Feng; Osaka, Tetsuya.

In: Nano Biomedicine and Engineering, Vol. 2, No. 1, 2010, p. 45-55.

Research output: Contribution to journalArticle

Cui, D, Li, Q, Huang, P, Wang, K, Kong, Y, Zhang, H, You, X, He, R, Song, H, Wang, J, Bao, C, Asahi, T, Gao, F & Osaka, T 2010, 'Real time PCR based on fluorescent quenching of mercaptoacetic acid-modified cdte quantum dots for ultrasensitive specific detection of nucleic acids', Nano Biomedicine and Engineering, vol. 2, no. 1, pp. 45-55.
Cui, Daxiang ; Li, Qing ; Huang, Peng ; Wang, Kan ; Kong, Yifei ; Zhang, Hong ; You, Xiaogang ; He, Rong ; Song, Hua ; Wang, Jingping ; Bao, Chenchen ; Asahi, Toru ; Gao, Feng ; Osaka, Tetsuya. / Real time PCR based on fluorescent quenching of mercaptoacetic acid-modified cdte quantum dots for ultrasensitive specific detection of nucleic acids. In: Nano Biomedicine and Engineering. 2010 ; Vol. 2, No. 1. pp. 45-55.
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