Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity

Y. Shirasaki, M. Yamagishi, K. Izawa, K. Nakagawa, A. Nakahara, N. Suzuki, J. Mizuno, T. Sekiguchi, T. Heike, R. Nishikomori, S. Shoji, O. Ohara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Live-cell imaging of inflammatory cytokine secretion from human monocytes was successfully performed on a microfabricated well array chip by time-resolved fluoroimmunoassay using total internal reflection fluorescence microscopy. The system enabled to monitor secretion activity from tens of cells at a time resolution of 1 min. We first observed the close correlation between the loss of cell membrane integrity and "non-classical secretion" of IL-1β cytokine of inflammasome activated human peripheral monocytes.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1686-1688
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Keywords

  • Real-time secretion analysis
  • Single cell
  • TIRF

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Shirasaki, Y., Yamagishi, M., Izawa, K., Nakagawa, K., Nakahara, A., Suzuki, N., Mizuno, J., Sekiguchi, T., Heike, T., Nishikomori, R., Shoji, S., & Ohara, O. (2013). Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1686-1688). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3). Chemical and Biological Microsystems Society.