Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity

Y. Shirasaki, M. Yamagishi, K. Izawa, K. Nakagawa, A. Nakahara, N. Suzuki, Jun Mizuno, Tetsushi Sekiguchi, T. Heike, R. Nishikomori, Shuichi Shoji, O. Ohara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Live-cell imaging of inflammatory cytokine secretion from human monocytes was successfully performed on a microfabricated well array chip by time-resolved fluoroimmunoassay using total internal reflection fluorescence microscopy. The system enabled to monitor secretion activity from tens of cells at a time resolution of 1 min. We first observed the close correlation between the loss of cell membrane integrity and "non-classical secretion" of IL-1β cytokine of inflammasome activated human peripheral monocytes.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1686-1688
Number of pages3
Volume3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg
Duration: 2013 Oct 272013 Oct 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Cell membranes
Interleukin-1
Inflammasomes
Cytokines
Fluorescence microscopy
Imaging techniques

Keywords

  • Real-time secretion analysis
  • Single cell
  • TIRF

ASJC Scopus subject areas

  • Bioengineering

Cite this

Shirasaki, Y., Yamagishi, M., Izawa, K., Nakagawa, K., Nakahara, A., Suzuki, N., ... Ohara, O. (2013). Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 3, pp. 1686-1688). Chemical and Biological Microsystems Society.

Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity. / Shirasaki, Y.; Yamagishi, M.; Izawa, K.; Nakagawa, K.; Nakahara, A.; Suzuki, N.; Mizuno, Jun; Sekiguchi, Tetsushi; Heike, T.; Nishikomori, R.; Shoji, Shuichi; Ohara, O.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3 Chemical and Biological Microsystems Society, 2013. p. 1686-1688.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shirasaki, Y, Yamagishi, M, Izawa, K, Nakagawa, K, Nakahara, A, Suzuki, N, Mizuno, J, Sekiguchi, T, Heike, T, Nishikomori, R, Shoji, S & Ohara, O 2013, Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 3, Chemical and Biological Microsystems Society, pp. 1686-1688, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, 13/10/27.
Shirasaki Y, Yamagishi M, Izawa K, Nakagawa K, Nakahara A, Suzuki N et al. Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3. Chemical and Biological Microsystems Society. 2013. p. 1686-1688
Shirasaki, Y. ; Yamagishi, M. ; Izawa, K. ; Nakagawa, K. ; Nakahara, A. ; Suzuki, N. ; Mizuno, Jun ; Sekiguchi, Tetsushi ; Heike, T. ; Nishikomori, R. ; Shoji, Shuichi ; Ohara, O. / Real-time secretion analysis revealed correlation of IL-1β release and loss of cell membrane integrity. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 3 Chemical and Biological Microsystems Society, 2013. pp. 1686-1688
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