Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors

Masao Kobayashi; Joseph H. Laver; Takashi Kato; Hiroshi Miyazaki; Makio Ogawa

doi:10.1182/blood.v86.7.2494.2494

Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors

Masao Kobayashi, Joseph H. Laver, Takashi Kato, Hiroshi Miyazaki, Makio Ogawa

School of Education

Research output: Contribution to journal › Article

152 Citations (Scopus)

Abstract

We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34⁺ bone marrow and cord blood cells. The burst- promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less than that of interleukin-3 but was comparable with that of granulocyte/macrophage colony- stimulating factor and steel factor. The soluble form of Mpl receptor inhibited burst enhancing effects of TPO, suggesting that the BPA effects of TPO are mediated through the Mpl receptor. These results further delineate the physiologic roles of TPO and may aid in the determination of the clinical usages of TPO.

Original language	English
Pages (from-to)	2494-2499
Number of pages	6
Journal	Blood
Volume	86
Issue number	7
DOIs	https://doi.org/10.1182/blood.v86.7.2494.2494
Publication status	Published - 1995 Oct 1

ASJC Scopus subject areas

Biochemistry
Immunology
Hematology
Cell Biology

Access to Document

10.1182/blood.v86.7.2494.2494

Fingerprint Dive into the research topics of 'Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors'. Together they form a unique fingerprint.

Cite this

Kobayashi, M., Laver, J. H., Kato, T., Miyazaki, H., & Ogawa, M. (1995). Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors. Blood, 86(7), 2494-2499. https://doi.org/10.1182/blood.v86.7.2494.2494

@article{8bf75485bb494d5faf960effee8f20cf,

title = "Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors",

abstract = "We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34+ bone marrow and cord blood cells. The burst- promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less than that of interleukin-3 but was comparable with that of granulocyte/macrophage colony- stimulating factor and steel factor. The soluble form of Mpl receptor inhibited burst enhancing effects of TPO, suggesting that the BPA effects of TPO are mediated through the Mpl receptor. These results further delineate the physiologic roles of TPO and may aid in the determination of the clinical usages of TPO.",

author = "Masao Kobayashi and Laver, {Joseph H.} and Takashi Kato and Hiroshi Miyazaki and Makio Ogawa",

year = "1995",

month = oct,

day = "1",

doi = "10.1182/blood.v86.7.2494.2494",

language = "English",

volume = "86",

pages = "2494--2499",

journal = "Blood",

issn = "0006-4971",

publisher = "American Society of Hematology",

number = "7",

}

TY - JOUR

T1 - Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors

AU - Kobayashi, Masao

AU - Laver, Joseph H.

AU - Kato, Takashi

AU - Miyazaki, Hiroshi

AU - Ogawa, Makio

PY - 1995/10/1

Y1 - 1995/10/1

N2 - We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34+ bone marrow and cord blood cells. The burst- promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less than that of interleukin-3 but was comparable with that of granulocyte/macrophage colony- stimulating factor and steel factor. The soluble form of Mpl receptor inhibited burst enhancing effects of TPO, suggesting that the BPA effects of TPO are mediated through the Mpl receptor. These results further delineate the physiologic roles of TPO and may aid in the determination of the clinical usages of TPO.

AB - We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34+ bone marrow and cord blood cells. The burst- promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less than that of interleukin-3 but was comparable with that of granulocyte/macrophage colony- stimulating factor and steel factor. The soluble form of Mpl receptor inhibited burst enhancing effects of TPO, suggesting that the BPA effects of TPO are mediated through the Mpl receptor. These results further delineate the physiologic roles of TPO and may aid in the determination of the clinical usages of TPO.

UR - http://www.scopus.com/inward/record.url?scp=0029027918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029027918&partnerID=8YFLogxK

U2 - 10.1182/blood.v86.7.2494.2494

DO - 10.1182/blood.v86.7.2494.2494

M3 - Article

C2 - 7545458

AN - SCOPUS:0029027918

VL - 86

SP - 2494

EP - 2499

JO - Blood

JF - Blood

SN - 0006-4971

IS - 7

ER -