TY - JOUR
T1 - Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors
AU - Kobayashi, Masao
AU - Laver, Joseph H.
AU - Kato, Takashi
AU - Miyazaki, Hiroshi
AU - Ogawa, Makio
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1995/10/1
Y1 - 1995/10/1
N2 - We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34+ bone marrow and cord blood cells. The burst- promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less than that of interleukin-3 but was comparable with that of granulocyte/macrophage colony- stimulating factor and steel factor. The soluble form of Mpl receptor inhibited burst enhancing effects of TPO, suggesting that the BPA effects of TPO are mediated through the Mpl receptor. These results further delineate the physiologic roles of TPO and may aid in the determination of the clinical usages of TPO.
AB - We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34+ bone marrow and cord blood cells. The burst- promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less than that of interleukin-3 but was comparable with that of granulocyte/macrophage colony- stimulating factor and steel factor. The soluble form of Mpl receptor inhibited burst enhancing effects of TPO, suggesting that the BPA effects of TPO are mediated through the Mpl receptor. These results further delineate the physiologic roles of TPO and may aid in the determination of the clinical usages of TPO.
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U2 - 10.1182/blood.v86.7.2494.2494
DO - 10.1182/blood.v86.7.2494.2494
M3 - Article
C2 - 7545458
AN - SCOPUS:0029027918
VL - 86
SP - 2494
EP - 2499
JO - Blood
JF - Blood
SN - 0006-4971
IS - 7
ER -