Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro

Masato Nakajima, Keiko Imai, Hiroshi Ito, Taeko Nishiwaki, Yoriko Murayama, Hideo Iwasaki, Tokitaka Oyama, Takao Kondo

Research output: Contribution to journalArticle

648 Citations (Scopus)

Abstract

Kai proteins globally regulate circadian gene expression of cyanobacteria. The KaiC phosphorylation cycle, which persists even without transcription or translation, is assumed to be a basic timing process of the circadian clock. We have reconstituted the self-sustainable oscillation of KaiC phosphorylation in vitro by incubating KaiC with KaiA, KaiB, and adenosine triphosphate. The period of the in vitro oscillation was stable despite temperature change (temperature compensation), and the circadian periods observed in vivo in KaiC mutant strains were consistent with those measured in vitro. The enigma of the circadian clock can now be studied in vitro by examining the interactions between three Kai proteins.

Original languageEnglish
Pages (from-to)414-415
Number of pages2
JournalScience
Volume308
Issue number5720
DOIs
Publication statusPublished - 2005 Apr 15
Externally publishedYes

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Phosphorylation
Circadian Clocks
Temperature
Cyanobacteria
Proteins
Adenosine Triphosphate
Gene Expression
In Vitro Techniques

ASJC Scopus subject areas

  • General

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Nakajima, M., Imai, K., Ito, H., Nishiwaki, T., Murayama, Y., Iwasaki, H., ... Kondo, T. (2005). Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro. Science, 308(5720), 414-415. https://doi.org/10.1126/science.1108451

Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro. / Nakajima, Masato; Imai, Keiko; Ito, Hiroshi; Nishiwaki, Taeko; Murayama, Yoriko; Iwasaki, Hideo; Oyama, Tokitaka; Kondo, Takao.

In: Science, Vol. 308, No. 5720, 15.04.2005, p. 414-415.

Research output: Contribution to journalArticle

Nakajima, M, Imai, K, Ito, H, Nishiwaki, T, Murayama, Y, Iwasaki, H, Oyama, T & Kondo, T 2005, 'Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro', Science, vol. 308, no. 5720, pp. 414-415. https://doi.org/10.1126/science.1108451
Nakajima, Masato ; Imai, Keiko ; Ito, Hiroshi ; Nishiwaki, Taeko ; Murayama, Yoriko ; Iwasaki, Hideo ; Oyama, Tokitaka ; Kondo, Takao. / Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro. In: Science. 2005 ; Vol. 308, No. 5720. pp. 414-415.
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