Reconstructed ancestral enzymes suggest long-Term cooling of Earth's photic zone since the Archean

Amanda K. Garcia, J. William Schopf, Shin Ichi Yokobori, Satoshi Akanuma, Akihiko Yamagishi

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Paleotemperatures inferred from the isotopic compositions (δ18O and δ30Si) of marine cherts suggest that Earth's oceans cooled from 70 ± 15 °C in the Archean to the present ∼15 °C. This interpretation, however, has been subject to question due to uncertainties regarding oceanic isotopic compositions, diagenetic or metamorphic resetting of the isotopic record, and depositional environments. Analyses of the thermostability of reconstructed ancestral enzymes provide an independent method by which to assess the temperature history inferred from the isotopic evidence. Although previous studies have demonstrated extreme thermostability in reconstructed archaeal and bacterial proteins compatible with a hot early Earth, taxa investigated may have inhabited local thermal environments that differed significantly from average surface conditions. We here present thermostability measurements of reconstructed ancestral enzymatically active nucleoside diphosphate kinases (NDKs) derived from light-requiring prokaryotic and eukaryotic phototrophs having widely separated fossil-based divergence ages. The ancestral environmental temperatures thereby determined for these photic-zone organisms-shown in modern taxa to correlate strongly with NDK thermostability-Are inferred to reflect ancient surface-environment paleotemperatures. Our results suggest that Earth's surface temperature decreased over geological time from ∼65-80 °C in the Archean, a finding consistent both with previous isotope-based and protein reconstruction-based interpretations. Interdisciplinary studies such as those reported here integrating genomic, geologic, and paleontologic data hold promise for providing new insight into the coevolution of life and environment over Earth history.

    Original languageEnglish
    Pages (from-to)4619-4624
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume114
    Issue number18
    DOIs
    Publication statusPublished - 2017 May 2

    Fingerprint

    Nucleoside-Diphosphate Kinase
    Enzymes
    Temperature
    Archaeal Proteins
    Interdisciplinary Studies
    Bacterial Proteins
    Oceans and Seas
    Isotopes
    Uncertainty
    Hot Temperature
    History
    Light
    Proteins

    Keywords

    • Ancestral sequence reconstruction
    • Enzyme thermostability
    • Nucleoside diphosphate kinase
    • Phototroph
    • Precambrian

    ASJC Scopus subject areas

    • General

    Cite this

    Reconstructed ancestral enzymes suggest long-Term cooling of Earth's photic zone since the Archean. / Garcia, Amanda K.; Schopf, J. William; Yokobori, Shin Ichi; Akanuma, Satoshi; Yamagishi, Akihiko.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 18, 02.05.2017, p. 4619-4624.

    Research output: Contribution to journalArticle

    Garcia, Amanda K. ; Schopf, J. William ; Yokobori, Shin Ichi ; Akanuma, Satoshi ; Yamagishi, Akihiko. / Reconstructed ancestral enzymes suggest long-Term cooling of Earth's photic zone since the Archean. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 18. pp. 4619-4624.
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