Reconstruction of 3D stacked hepatocyte tissues using degradable, microporous poly(d,l-lactide-co-glycolide) membranes

Junichi Kasuya, Ryo Sudo, Ryu Tamogami, Genta Masuda, Toshihiro Mitaka, Mariko Ikeda, Kazuo Tanishita

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

There is great demand for constructing well-organized three-dimensional (3D) tissues invitro. Here, we developed a 3D stacked culture method using biodegradable poly(d,l-lactide-co- glycolide) (PLGA) membranes with defined topography. Pore size and porosity of the membranes can be controlled by changing the moisture content during fabrication. The optimized membrane served as a scaffold to manipulate small hepatocyte (SH) layers when they were stacked, while it degraded after stacking, resulting in the reorganization of the cells into a 3D stacked structure. Immunofluorescent staining for domain markers of cell polarity and electron microscopy confirmed that the cells in the 3D stacked structures recovered polarity. Furthermore, the cells exhibited improved liver-specific function as compared with cells in a monolayer. This 3D stacked culture may enable reconstruction of multilayered hepatic tissues with highly differentiated functions invitro.

Original languageEnglish
Pages (from-to)2693-2700
Number of pages8
JournalBiomaterials
Volume33
Issue number9
DOIs
Publication statusPublished - 2012 Mar
Externally publishedYes

Keywords

  • Biodegradation
  • Cell culture
  • Hepatocyte
  • Liver
  • Polyglycolic acid
  • Polylactic acid

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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