Reconstruction of a Vascular Bed with Perfusable Blood Vessels Using a Decellularized Porcine Small Intestine for Clinical Application

Yusuke Tobe, Katsuhisa Sakaguchi, Jun Homma, Kazunori Sano, Eiji Kobayashi, Hidekazu Sekine, Kiyotaka Iwasaki, Tatsuya Shimizu, Mitsuo Umezu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Engineering three-dimensional tissues in vitro for regenerative therapy is highly desired. Vascular beds with connectable arteries and veins play crucial roles in fabricating three-dimensional tissues in vitro and improving the survival rate of transplanted three-dimensional tissues in vivo. Here, we developed a method to reconstruct a new vascular bed that could be implanted into humans for clinical applications. Porcine small intestine with an arteriovenous loop was selected as a vascular bed skeleton and decellularized using transvascular perfusion with both detergent and enzyme. Subsequently, the decellularized intestinal graft was re-endothelialized with human cells to allow blood perfusion into the vascular bed. The small intestinal graft was successfully decellularized without severe damage to the burst pressure of the graft, and the decellularized intestinal graft could be transplanted without a severe inflammatory response in rats for two weeks. In addition, the decellularized intestinal graft was partially vascularized with human endothelial cells in seven days. Therefore, the reconstructed intestinal vascular bed may serve as a human transplantable vascular bed with potential in clinical investigations.

Original languageEnglish
Title of host publication11th Asian-Pacific Conference on Medical and Biological Engineering - Proceedings of the Online Conference, APCMBE 2020
EditorsYasuyuki Shiraishi, Ichiro Sakuma, Keiji Naruse, Akinori Ueno
PublisherSpringer Science and Business Media Deutschland GmbH
Pages284-292
Number of pages9
ISBN (Print)9783030661687
DOIs
Publication statusPublished - 2021
Event11th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2020 - Okayama, Japan
Duration: 2020 May 252020 May 27

Publication series

NameIFMBE Proceedings
Volume82
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277

Conference

Conference11th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2020
CountryJapan
CityOkayama
Period20/5/2520/5/27

Keywords

  • Decellularization
  • Vascular bed
  • Vascularization

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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