Reconstruction of tissue and gene expression

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Cell-cell communication between multiple cell populations is believed to be important for the activation of many cellular functions. It has previously been reported that rat hepatocytes in a double layer co-culture system with human endothelial cells were able to maintain albumin expression for a prolonged period of time. This result suggests that essential functions of hepatocytes were maintained via communication with endothelial cells in this co-culture system. In order to analyze the hepatocyte gene expression profile of double-layered co-culture, we try to isolate cDNAs, which increase in this co-culture system, from a subtractive library constructed by suppression subtractive hybridization (SSH). The results indicate that the many hepatocyte functions are increased by layered with HUVEC.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Number of pages2
Publication statusPublished - 2006
Externally publishedYes
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: 2006 Sep 202006 Sep 22


Other55th Society of Polymer Science Japan Symposium on Macromolecules



  • Cell sheet
  • Co-culture
  • Gene expression
  • Re-construction of liver

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Taniguchi, A. (2006). Reconstruction of tissue and gene expression. In Polymer Preprints, Japan (2 ed., Vol. 55, pp. 5347-5348)