Recovery of a marine cyanobacterial recombinant product using fish-feed organisms

Koji Sode, Toshihiro Hayashi, Masahiro Tatara, Naoaki Hatano, Hiromi Yoshida, Haruko Takeyama, Takashi Oshiro, Tadashi Matsunaga

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A novel approach for the recovery of recombinant products expressed in marine cyanobacteria, utilizing the fish-feed organism, brine shrimp (Artemia salina), is presented. Chloramphenicol acetyltransferase (CAT) was highly expressed in the marine unicellular cyanobacterium, Synechococcus sp. NKBG15041c, under a c-phycocyanin promoter derived from Synechococcus sp. PCC7942. These recombinant marine cyanobacteria were recovered using brine shrimp. CAT activity was retained in brine shrimp and increased with the time they were fed with recombinant cyanobacteria expressing CAT. Subsequently, the brine shrimp were fed to medaka fish. About 80% of added CAT was observed in the digestive organs of medaka for more than 9 h after feeding. These results demonstrate a novel process that can deliver a recombinant product to fish via fish-feed organisms without any purification steps.

Original languageEnglish
Pages (from-to)82-86
Number of pages5
JournalJournal of Marine Biotechnology
Volume4
Issue number2
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Fish products
Fish Products
Artemia
Chloramphenicol O-Acetyltransferase
Fish
Cyanobacteria
Recovery
Oryzias
Synechococcus
Fishes
Phycocyanin
Purification
brine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sode, K., Hayashi, T., Tatara, M., Hatano, N., Yoshida, H., Takeyama, H., ... Matsunaga, T. (1996). Recovery of a marine cyanobacterial recombinant product using fish-feed organisms. Journal of Marine Biotechnology, 4(2), 82-86.

Recovery of a marine cyanobacterial recombinant product using fish-feed organisms. / Sode, Koji; Hayashi, Toshihiro; Tatara, Masahiro; Hatano, Naoaki; Yoshida, Hiromi; Takeyama, Haruko; Oshiro, Takashi; Matsunaga, Tadashi.

In: Journal of Marine Biotechnology, Vol. 4, No. 2, 1996, p. 82-86.

Research output: Contribution to journalArticle

Sode, K, Hayashi, T, Tatara, M, Hatano, N, Yoshida, H, Takeyama, H, Oshiro, T & Matsunaga, T 1996, 'Recovery of a marine cyanobacterial recombinant product using fish-feed organisms', Journal of Marine Biotechnology, vol. 4, no. 2, pp. 82-86.
Sode, Koji ; Hayashi, Toshihiro ; Tatara, Masahiro ; Hatano, Naoaki ; Yoshida, Hiromi ; Takeyama, Haruko ; Oshiro, Takashi ; Matsunaga, Tadashi. / Recovery of a marine cyanobacterial recombinant product using fish-feed organisms. In: Journal of Marine Biotechnology. 1996 ; Vol. 4, No. 2. pp. 82-86.
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