Recruitment of a species-specific translational arrest module to monitor different cellular processes

Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, Koreaki Ito

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Nascent chain-mediated translation arrest serves as a mechanism of gene regulation. A class of regulatory nascent polypeptides undergoes elongation arrest in manners controlled by the dynamic behavior of the growing chain; Escherichia coli SecM monitors the Sec protein export pathway and Bacillus subtilis MifM monitors the YidC membrane protein integration/folding pathway. We show that MifM and SecM interact with the ribosome in a speciesspecific manner to stall only the ribosome from the homologous species. Despite this specificity, MifM is not exclusively designed to monitor membrane protein integration because it can be converted into a secretion monitor by replacing the N-terminal transmembrane sequence with a secretion signal sequence. These results show that a regulatory nascent chain is composed of two modular elements, one devoted to elongation arrest and another devoted to subcellular targeting, and they imply that physical pulling force generated by the latter triggers release of the arrest executed by the former. The combinatorial nature may assure common occurrence of nascent chain-mediated regulation.

Original languageEnglish
Pages (from-to)6073-6078
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number15
DOIs
Publication statusPublished - 2011 Apr 12
Externally publishedYes

Fingerprint

Ribosomes
Membrane Proteins
Protein Folding
Protein Sorting Signals
Bacillus subtilis
Escherichia coli
Peptides
Genes
Proteins

Keywords

  • Exit tunnel
  • SecA
  • SpoIIIJ
  • Stalling sequence

ASJC Scopus subject areas

  • General

Cite this

Recruitment of a species-specific translational arrest module to monitor different cellular processes. / Chiba, Shinobu; Kanamori, Takashi; Ueda, Takuya; Akiyama, Yoshinori; Pogliano, Kit; Ito, Koreaki.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 15, 12.04.2011, p. 6073-6078.

Research output: Contribution to journalArticle

Chiba, Shinobu ; Kanamori, Takashi ; Ueda, Takuya ; Akiyama, Yoshinori ; Pogliano, Kit ; Ito, Koreaki. / Recruitment of a species-specific translational arrest module to monitor different cellular processes. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 15. pp. 6073-6078.
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