Recycle use of Sphingomonas sp. CDH-7 cells for continuous degradation of carbazole in the presence of MgCl2

Hiroyuki Nakagawa, Kotaro Kirimura, Takako Nitta, Kuniki Kino, Ryuichiro Kurane, Shoji Usami

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Carbazole (CA) is a heterocyclic nitrogen compound contained in the crude petroleum oil and recalcitrant to removal through the refinery processes. For development of the efficient CA-degradation bioprocess, conditions for the recycle use of Sphingomonas sp. CDH-7 resting cells were examined. When the resting cells (O.D.660 3.3) were shaken in 50 mm K2HPO4-KH2PO4 buffer (pH 7.0) containing CA 1000 mg/L, CA 880 mg/L was degraded within 3 h, but thereafter the activity decreased markedly. However, the activity was found to be restored to the initial level after the shaking treatment for 3 h in CA-free medium solution or in the buffer containing 20 mM MgCl2. Although the CA-degradation activity of CDH-7 resting cells was lost after 3 h of shaking in the buffer containing 100 mM EDTA, it was restored through the shaking treatment for 3 h in the buffer containing 20 mM MgCl2. When CA was periodically added eight times at a concentration of 100 mg/L (0.599 mM) to the reaction mixture containing the resting cells, CA 778 mg/L (4.66 mM) was continuously degraded within 35 h by the recycle use of resting cells, with the restoration treatment after each CA-degradation reaction by the resting cells.

    Original languageEnglish
    Pages (from-to)251-256
    Number of pages6
    JournalCurrent Microbiology
    Volume44
    Issue number4
    DOIs
    Publication statusPublished - 2002 Apr 1

    Fingerprint

    Sphingomonas
    Magnesium Chloride
    Buffers
    Petroleum
    Nitrogen Compounds
    Heterocyclic Compounds
    carbazole
    Edetic Acid

    ASJC Scopus subject areas

    • Microbiology

    Cite this

    Recycle use of Sphingomonas sp. CDH-7 cells for continuous degradation of carbazole in the presence of MgCl2 . / Nakagawa, Hiroyuki; Kirimura, Kotaro; Nitta, Takako; Kino, Kuniki; Kurane, Ryuichiro; Usami, Shoji.

    In: Current Microbiology, Vol. 44, No. 4, 01.04.2002, p. 251-256.

    Research output: Contribution to journalArticle

    Nakagawa, Hiroyuki ; Kirimura, Kotaro ; Nitta, Takako ; Kino, Kuniki ; Kurane, Ryuichiro ; Usami, Shoji. / Recycle use of Sphingomonas sp. CDH-7 cells for continuous degradation of carbazole in the presence of MgCl2 In: Current Microbiology. 2002 ; Vol. 44, No. 4. pp. 251-256.
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