Reduced food anticipatory activity in genetically orexin (hypocretin) neuron-ablated mice

Masashi Akiyama, Tomoyo Yuasa, Naomi Hayasaka, Kazumasa Horikawa, Takeshi Sakurai, Shigenobu Shibata

    Research output: Contribution to journalArticle

    144 Citations (Scopus)

    Abstract

    Daily restricted feeding (RF) produces an anticipatory locomotor activity rhythm and entrains the peripheral molecular oscillator independently of the central pacemaker located in the suprachiasmatic nucleus (SCN). As orexins (hypocretins) are neuropeptides that coordinate sleep/wake patterns and motivated behaviours, such as food seeking, we studied the involvement of orexin in the food anticipatory activity (FAA) induced by RF. Daily RF shifted the mRNA rhythm of a clock-controlled gene mDbp in the cerebral cortex and caudate putamen but not in the SCN. Under these experimental conditions, prepro-orexin mRNA and orexin A immunoreactivity in the lateral hypothalamic area (LHA) did not show daily variation. Fasting increased the number of orexin A-ir cells, while RF did not. However, RF shifted the peak of Fos expression of the orexin neurons from night to day. Genetic ablation of orexin neurons in orexin/ataxin-3 transgenic mice severely reduced the formation of FAA under RF conditions. The expression of mNpas2 mRNA, a transcription factor thought to be involved in regulation of the food entrainable oscillator as well as mPer1 and mBmal1 mRNA, was reduced in the forebrain of orexin/ataxin-3 mice. Based on these results, we suggest that activity of the orexin neuron in the LHA contributes to the promotion and maintenance of FAA.

    Original languageEnglish
    Pages (from-to)3054-3062
    Number of pages9
    JournalEuropean Journal of Neuroscience
    Volume20
    Issue number11
    DOIs
    Publication statusPublished - 2004 Dec

    Fingerprint

    Neurons
    Food
    Lateral Hypothalamic Area
    Messenger RNA
    Suprachiasmatic Nucleus
    Putamen
    Locomotion
    Prosencephalon
    Neuropeptides
    Cerebral Cortex
    Transgenic Mice
    Fasting
    Sleep
    Transcription Factors
    Maintenance
    Orexins
    Genes
    Ataxin-3

    Keywords

    • Food anticipatory activity
    • Hypocretin
    • Lateral hypothalamus
    • Orexin
    • Restricted feeding

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Reduced food anticipatory activity in genetically orexin (hypocretin) neuron-ablated mice. / Akiyama, Masashi; Yuasa, Tomoyo; Hayasaka, Naomi; Horikawa, Kazumasa; Sakurai, Takeshi; Shibata, Shigenobu.

    In: European Journal of Neuroscience, Vol. 20, No. 11, 12.2004, p. 3054-3062.

    Research output: Contribution to journalArticle

    Akiyama, Masashi ; Yuasa, Tomoyo ; Hayasaka, Naomi ; Horikawa, Kazumasa ; Sakurai, Takeshi ; Shibata, Shigenobu. / Reduced food anticipatory activity in genetically orexin (hypocretin) neuron-ablated mice. In: European Journal of Neuroscience. 2004 ; Vol. 20, No. 11. pp. 3054-3062.
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