Reduction in formation of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP)-induced aberrant crypt foci in the rat colon by docosahexaenoic acid (DHA)

Mami Takahashi, Yukari Totsuka, Mitsuharu Masuda, Kazunori Fukuda, Atsuko Oguri, Kazunaga Yazawa, Takashi Sugimura, Keiji Wakabayashi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Docosahexaenoic acid (DHA), a major component of fish oil, suppresses the formation and growth of aberrant crypt foci induced by 1,2-dimethylhydrazine and azoxymethane. In the present study we examined the effects of intragastric gavage administration of DHA on the yield of rat colonic aberrant crypt foci due to treatment with a heterocyclic amine, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), which induces colon cancer in male F344 rats and is considered to be a possible human colon carcinogen. Male F344 rats were given a standard diet (AIN-76A) and received 10 doses of PhIP (75 mg/kg body wt, by intragastric intubation, on days 1-5 and 8-12) with or without intragastric application of 1 ml DHA 4 h prior to each carcinogen treatment, followed by further DHA dosing. The numbers of PhIP-induced aberrant crypt foci per colon after 4 and 12 weeks DHA administration were significantly reduced to 47 and 38% respectively of the values obtained when PhIP alone was used. The mean number of aberrant crypts per focus was also decreased by DHA treatment. At week 4 the PhIP-DNA adduct levels in the colon of rats from the PhIP+DHA group were approximately two thirds of the PhIP group value. The results thus suggest that DHA exerts a preventive effect on PhIP-induced colon carcinogenesis.

Original languageEnglish
Pages (from-to)1937-1941
Number of pages5
JournalCarcinogenesis
Volume18
Issue number10
DOIs
Publication statusPublished - 1997 Oct
Externally publishedYes

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Aberrant Crypt Foci
Docosahexaenoic Acids
Colon
Inbred F344 Rats
Carcinogens
Azoxymethane
1,2-Dimethylhydrazine
2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine
Fish Oils
Intubation
Colonic Neoplasms
Amines
Carcinogenesis
Therapeutics
Diet

ASJC Scopus subject areas

  • Cancer Research

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Reduction in formation of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP)-induced aberrant crypt foci in the rat colon by docosahexaenoic acid (DHA). / Takahashi, Mami; Totsuka, Yukari; Masuda, Mitsuharu; Fukuda, Kazunori; Oguri, Atsuko; Yazawa, Kazunaga; Sugimura, Takashi; Wakabayashi, Keiji.

In: Carcinogenesis, Vol. 18, No. 10, 10.1997, p. 1937-1941.

Research output: Contribution to journalArticle

Takahashi, M, Totsuka, Y, Masuda, M, Fukuda, K, Oguri, A, Yazawa, K, Sugimura, T & Wakabayashi, K 1997, 'Reduction in formation of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP)-induced aberrant crypt foci in the rat colon by docosahexaenoic acid (DHA)', Carcinogenesis, vol. 18, no. 10, pp. 1937-1941. https://doi.org/10.1093/carcin/18.10.1937
Takahashi, Mami ; Totsuka, Yukari ; Masuda, Mitsuharu ; Fukuda, Kazunori ; Oguri, Atsuko ; Yazawa, Kazunaga ; Sugimura, Takashi ; Wakabayashi, Keiji. / Reduction in formation of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP)-induced aberrant crypt foci in the rat colon by docosahexaenoic acid (DHA). In: Carcinogenesis. 1997 ; Vol. 18, No. 10. pp. 1937-1941.
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abstract = "Docosahexaenoic acid (DHA), a major component of fish oil, suppresses the formation and growth of aberrant crypt foci induced by 1,2-dimethylhydrazine and azoxymethane. In the present study we examined the effects of intragastric gavage administration of DHA on the yield of rat colonic aberrant crypt foci due to treatment with a heterocyclic amine, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), which induces colon cancer in male F344 rats and is considered to be a possible human colon carcinogen. Male F344 rats were given a standard diet (AIN-76A) and received 10 doses of PhIP (75 mg/kg body wt, by intragastric intubation, on days 1-5 and 8-12) with or without intragastric application of 1 ml DHA 4 h prior to each carcinogen treatment, followed by further DHA dosing. The numbers of PhIP-induced aberrant crypt foci per colon after 4 and 12 weeks DHA administration were significantly reduced to 47 and 38{\%} respectively of the values obtained when PhIP alone was used. The mean number of aberrant crypts per focus was also decreased by DHA treatment. At week 4 the PhIP-DNA adduct levels in the colon of rats from the PhIP+DHA group were approximately two thirds of the PhIP group value. The results thus suggest that DHA exerts a preventive effect on PhIP-induced colon carcinogenesis.",
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