Reduction of methemoglobin via electron transfer from photoreduced flavin: Restoration of O2-binding of concentrated hemoglobin solution coencapsulated in phospholipid vesicles

Hiromi Sakai, Yohei Masada, Hiroto Onuma, Shinji Takeoka, Eishun Tsuchida

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Ferric methemoglobin is reduced to its ferrous form by photoirradiation either by direct photoexcitation of the heme portion to induce electron transfer from the surrounding media (Sakai at al. (2000) Biochemistry 39, 14595-14602) or by an indirect electron transfer from a photochemically reduced electron mediator such as flavin. In this research, we studied the mechanism and optimal condition that facilitates photoreduction of flavin mononucleotide (FMN) to FMNH2 by irradiation of visible light, and the succeeding reduction of concentrated metHb in phospholipid vesicles to restore its O2 binding ability. Visible light irradiation (435 nm) of a metHb solution containing FMN and an electron donor such as EDTA showed a significantly fast reduction to ferrous Hb with a quantum yield (Φ) of 0.17, that is higher than the method of direct photoexcitation of heme (Φ = 0.006). Electron transfer from a donor molecule to metHb via FMN was completed within 30 ns. Native-PAGE and IEF electrophoresis indicated no chemical modification of the surface of the reduced Hb. Coencapsulation of concentrated Hb solution (35 g/dL) and the FMN/EDTA system in vesicles covered with a phospholipid bilayer membrane (Hb-vesicles, HbV, diameter: 250 nm) facilitated the metHb photoreduction even under aerobic conditions, and the reduced HbV restored the reversible O2 binding property. A concentrated HbV suspension ([Hb] = 8 g/dL) was sandwiched with two glass plates to form a liquid layer with the thickness of about 10 μ (close to capillary diameter in tissue, 5 μ), and visible light irradiation (221 mW/cm2) completed 100% metHb photoreduction within 20 s. The photoreduced FMNH2 reacted with O2 to produce H2O2, which was detected by the fluorescence measurement of the reaction of H2O2 and p-nitrophenylacetic acid. However, the amount of H2O2 generated during the photoreduction of HbV was significantly reduced in comparison with the homogeneous Hb solution, indicating that the photoreduced FMNH2 was effectively consumed during the metHb reduction in a highly concentrated condition inside the HbV nanoparticles.

    Original languageEnglish
    Pages (from-to)1037-1045
    Number of pages9
    JournalBioconjugate Chemistry
    Volume15
    Issue number5
    DOIs
    Publication statusPublished - 2004 Sep

    Fingerprint

    Methemoglobin
    Hemoglobin
    Phospholipids
    Flavin Mononucleotide
    Restoration
    Hemoglobins
    Electrons
    Photoexcitation
    Ethylenediaminetetraacetic acid
    Irradiation
    Heme
    Light
    Edetic Acid
    Native Polyacrylamide Gel Electrophoresis
    Biochemistry
    Chemical modification
    Quantum yield
    Electrophoresis
    Nanoparticles
    Glass

    ASJC Scopus subject areas

    • Chemistry(all)
    • Organic Chemistry
    • Clinical Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry

    Cite this

    Reduction of methemoglobin via electron transfer from photoreduced flavin : Restoration of O2-binding of concentrated hemoglobin solution coencapsulated in phospholipid vesicles. / Sakai, Hiromi; Masada, Yohei; Onuma, Hiroto; Takeoka, Shinji; Tsuchida, Eishun.

    In: Bioconjugate Chemistry, Vol. 15, No. 5, 09.2004, p. 1037-1045.

    Research output: Contribution to journalArticle

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    abstract = "Ferric methemoglobin is reduced to its ferrous form by photoirradiation either by direct photoexcitation of the heme portion to induce electron transfer from the surrounding media (Sakai at al. (2000) Biochemistry 39, 14595-14602) or by an indirect electron transfer from a photochemically reduced electron mediator such as flavin. In this research, we studied the mechanism and optimal condition that facilitates photoreduction of flavin mononucleotide (FMN) to FMNH2 by irradiation of visible light, and the succeeding reduction of concentrated metHb in phospholipid vesicles to restore its O2 binding ability. Visible light irradiation (435 nm) of a metHb solution containing FMN and an electron donor such as EDTA showed a significantly fast reduction to ferrous Hb with a quantum yield (Φ) of 0.17, that is higher than the method of direct photoexcitation of heme (Φ = 0.006). Electron transfer from a donor molecule to metHb via FMN was completed within 30 ns. Native-PAGE and IEF electrophoresis indicated no chemical modification of the surface of the reduced Hb. Coencapsulation of concentrated Hb solution (35 g/dL) and the FMN/EDTA system in vesicles covered with a phospholipid bilayer membrane (Hb-vesicles, HbV, diameter: 250 nm) facilitated the metHb photoreduction even under aerobic conditions, and the reduced HbV restored the reversible O2 binding property. A concentrated HbV suspension ([Hb] = 8 g/dL) was sandwiched with two glass plates to form a liquid layer with the thickness of about 10 μ (close to capillary diameter in tissue, 5 μ), and visible light irradiation (221 mW/cm2) completed 100{\%} metHb photoreduction within 20 s. The photoreduced FMNH2 reacted with O2 to produce H2O2, which was detected by the fluorescence measurement of the reaction of H2O2 and p-nitrophenylacetic acid. However, the amount of H2O2 generated during the photoreduction of HbV was significantly reduced in comparison with the homogeneous Hb solution, indicating that the photoreduced FMNH2 was effectively consumed during the metHb reduction in a highly concentrated condition inside the HbV nanoparticles.",
    author = "Hiromi Sakai and Yohei Masada and Hiroto Onuma and Shinji Takeoka and Eishun Tsuchida",
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    T2 - Restoration of O2-binding of concentrated hemoglobin solution coencapsulated in phospholipid vesicles

    AU - Sakai, Hiromi

    AU - Masada, Yohei

    AU - Onuma, Hiroto

    AU - Takeoka, Shinji

    AU - Tsuchida, Eishun

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    N2 - Ferric methemoglobin is reduced to its ferrous form by photoirradiation either by direct photoexcitation of the heme portion to induce electron transfer from the surrounding media (Sakai at al. (2000) Biochemistry 39, 14595-14602) or by an indirect electron transfer from a photochemically reduced electron mediator such as flavin. In this research, we studied the mechanism and optimal condition that facilitates photoreduction of flavin mononucleotide (FMN) to FMNH2 by irradiation of visible light, and the succeeding reduction of concentrated metHb in phospholipid vesicles to restore its O2 binding ability. Visible light irradiation (435 nm) of a metHb solution containing FMN and an electron donor such as EDTA showed a significantly fast reduction to ferrous Hb with a quantum yield (Φ) of 0.17, that is higher than the method of direct photoexcitation of heme (Φ = 0.006). Electron transfer from a donor molecule to metHb via FMN was completed within 30 ns. Native-PAGE and IEF electrophoresis indicated no chemical modification of the surface of the reduced Hb. Coencapsulation of concentrated Hb solution (35 g/dL) and the FMN/EDTA system in vesicles covered with a phospholipid bilayer membrane (Hb-vesicles, HbV, diameter: 250 nm) facilitated the metHb photoreduction even under aerobic conditions, and the reduced HbV restored the reversible O2 binding property. A concentrated HbV suspension ([Hb] = 8 g/dL) was sandwiched with two glass plates to form a liquid layer with the thickness of about 10 μ (close to capillary diameter in tissue, 5 μ), and visible light irradiation (221 mW/cm2) completed 100% metHb photoreduction within 20 s. The photoreduced FMNH2 reacted with O2 to produce H2O2, which was detected by the fluorescence measurement of the reaction of H2O2 and p-nitrophenylacetic acid. However, the amount of H2O2 generated during the photoreduction of HbV was significantly reduced in comparison with the homogeneous Hb solution, indicating that the photoreduced FMNH2 was effectively consumed during the metHb reduction in a highly concentrated condition inside the HbV nanoparticles.

    AB - Ferric methemoglobin is reduced to its ferrous form by photoirradiation either by direct photoexcitation of the heme portion to induce electron transfer from the surrounding media (Sakai at al. (2000) Biochemistry 39, 14595-14602) or by an indirect electron transfer from a photochemically reduced electron mediator such as flavin. In this research, we studied the mechanism and optimal condition that facilitates photoreduction of flavin mononucleotide (FMN) to FMNH2 by irradiation of visible light, and the succeeding reduction of concentrated metHb in phospholipid vesicles to restore its O2 binding ability. Visible light irradiation (435 nm) of a metHb solution containing FMN and an electron donor such as EDTA showed a significantly fast reduction to ferrous Hb with a quantum yield (Φ) of 0.17, that is higher than the method of direct photoexcitation of heme (Φ = 0.006). Electron transfer from a donor molecule to metHb via FMN was completed within 30 ns. Native-PAGE and IEF electrophoresis indicated no chemical modification of the surface of the reduced Hb. Coencapsulation of concentrated Hb solution (35 g/dL) and the FMN/EDTA system in vesicles covered with a phospholipid bilayer membrane (Hb-vesicles, HbV, diameter: 250 nm) facilitated the metHb photoreduction even under aerobic conditions, and the reduced HbV restored the reversible O2 binding property. A concentrated HbV suspension ([Hb] = 8 g/dL) was sandwiched with two glass plates to form a liquid layer with the thickness of about 10 μ (close to capillary diameter in tissue, 5 μ), and visible light irradiation (221 mW/cm2) completed 100% metHb photoreduction within 20 s. The photoreduced FMNH2 reacted with O2 to produce H2O2, which was detected by the fluorescence measurement of the reaction of H2O2 and p-nitrophenylacetic acid. However, the amount of H2O2 generated during the photoreduction of HbV was significantly reduced in comparison with the homogeneous Hb solution, indicating that the photoreduced FMNH2 was effectively consumed during the metHb reduction in a highly concentrated condition inside the HbV nanoparticles.

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