Abstract
An artificial material coated with a laminin-apatite composite layer on its surface would be useful as a percutaneous device with cell-adhesion properties and good biocompatibility. The present authors recently prepared such a composite by a liquid-phase coating process in which a surface-modified material was immersed in a laminin-containing calcium phosphate solution (LCP solution) supersaturated with respect to apatite. As a result of this coating process, however, the material increased in surface roughness, which may have an adverse effect on the prevention of bacterial infection. In the present study, a reduction of the surface roughness of the laminin-apatite composite layer was attempted by adding MgSO4 to the LCP solution as an inhibitor of apatite crystal growth. The surface roughness, Ra, of the resulting laminin-apatite composite layer decreased from approximately 0.43 to 0.31 μm upon increasing the MgSO4 concentration in the LCP solution from 0 to 1.5 mM, while retaining its cell adhesion characteristics. The thus-obtained material, having a laminin-apatite composite coating with reduced surface roughness, has potential as a material for percutaneous devices with improved resistance to bacterial infection through the interface between the device and the skin.
| Original language | English |
|---|---|
| Pages (from-to) | 1342-1348 |
| Number of pages | 7 |
| Journal | Acta Biomaterialia |
| Volume | 4 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2008 Sep |
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Keywords
- Apatite
- Inhibitory effect
- Laminin
- Magnesium
- Percutaneous device
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
- Biotechnology
- Biochemistry
- Molecular Biology
Cite this
Reduction of surface roughness of a laminin-apatite composite coating via inhibitory effect of magnesium ions on apatite crystal growth. / Oyane, Ayako; Ishikawa, Yuusuke; Yamazaki, Atsushi; Sogo, Yu; Furukawa, Katsuko; Ushida, Takashi; Ito, Atsuo.
In: Acta Biomaterialia, Vol. 4, No. 5, 09.2008, p. 1342-1348.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduction of surface roughness of a laminin-apatite composite coating via inhibitory effect of magnesium ions on apatite crystal growth
AU - Oyane, Ayako
AU - Ishikawa, Yuusuke
AU - Yamazaki, Atsushi
AU - Sogo, Yu
AU - Furukawa, Katsuko
AU - Ushida, Takashi
AU - Ito, Atsuo
PY - 2008/9
Y1 - 2008/9
N2 - An artificial material coated with a laminin-apatite composite layer on its surface would be useful as a percutaneous device with cell-adhesion properties and good biocompatibility. The present authors recently prepared such a composite by a liquid-phase coating process in which a surface-modified material was immersed in a laminin-containing calcium phosphate solution (LCP solution) supersaturated with respect to apatite. As a result of this coating process, however, the material increased in surface roughness, which may have an adverse effect on the prevention of bacterial infection. In the present study, a reduction of the surface roughness of the laminin-apatite composite layer was attempted by adding MgSO4 to the LCP solution as an inhibitor of apatite crystal growth. The surface roughness, Ra, of the resulting laminin-apatite composite layer decreased from approximately 0.43 to 0.31 μm upon increasing the MgSO4 concentration in the LCP solution from 0 to 1.5 mM, while retaining its cell adhesion characteristics. The thus-obtained material, having a laminin-apatite composite coating with reduced surface roughness, has potential as a material for percutaneous devices with improved resistance to bacterial infection through the interface between the device and the skin.
AB - An artificial material coated with a laminin-apatite composite layer on its surface would be useful as a percutaneous device with cell-adhesion properties and good biocompatibility. The present authors recently prepared such a composite by a liquid-phase coating process in which a surface-modified material was immersed in a laminin-containing calcium phosphate solution (LCP solution) supersaturated with respect to apatite. As a result of this coating process, however, the material increased in surface roughness, which may have an adverse effect on the prevention of bacterial infection. In the present study, a reduction of the surface roughness of the laminin-apatite composite layer was attempted by adding MgSO4 to the LCP solution as an inhibitor of apatite crystal growth. The surface roughness, Ra, of the resulting laminin-apatite composite layer decreased from approximately 0.43 to 0.31 μm upon increasing the MgSO4 concentration in the LCP solution from 0 to 1.5 mM, while retaining its cell adhesion characteristics. The thus-obtained material, having a laminin-apatite composite coating with reduced surface roughness, has potential as a material for percutaneous devices with improved resistance to bacterial infection through the interface between the device and the skin.
KW - Apatite
KW - Inhibitory effect
KW - Laminin
KW - Magnesium
KW - Percutaneous device
UR - http://www.scopus.com/inward/record.url?scp=48449092827&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48449092827&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2008.04.008
DO - 10.1016/j.actbio.2008.04.008
M3 - Article
C2 - 18485843
AN - SCOPUS:48449092827
VL - 4
SP - 1342
EP - 1348
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
IS - 5
ER -