Reduction of surface roughness of a laminin-apatite composite coating via inhibitory effect of magnesium ions on apatite crystal growth

Ayako Oyane, Yuusuke Ishikawa, Atsushi Yamazaki, Yu Sogo, Katsuko Furukawa, Takashi Ushida, Atsuo Ito

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An artificial material coated with a laminin-apatite composite layer on its surface would be useful as a percutaneous device with cell-adhesion properties and good biocompatibility. The present authors recently prepared such a composite by a liquid-phase coating process in which a surface-modified material was immersed in a laminin-containing calcium phosphate solution (LCP solution) supersaturated with respect to apatite. As a result of this coating process, however, the material increased in surface roughness, which may have an adverse effect on the prevention of bacterial infection. In the present study, a reduction of the surface roughness of the laminin-apatite composite layer was attempted by adding MgSO4 to the LCP solution as an inhibitor of apatite crystal growth. The surface roughness, Ra, of the resulting laminin-apatite composite layer decreased from approximately 0.43 to 0.31 μm upon increasing the MgSO4 concentration in the LCP solution from 0 to 1.5 mM, while retaining its cell adhesion characteristics. The thus-obtained material, having a laminin-apatite composite coating with reduced surface roughness, has potential as a material for percutaneous devices with improved resistance to bacterial infection through the interface between the device and the skin.

Original languageEnglish
Pages (from-to)1342-1348
Number of pages7
JournalActa Biomaterialia
Volume4
Issue number5
DOIs
Publication statusPublished - 2008 Sep 1

Keywords

  • Apatite
  • Inhibitory effect
  • Laminin
  • Magnesium
  • Percutaneous device

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

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