Abstract
An artificial material coated with a laminin-apatite composite layer on its surface would be useful as a percutaneous device with cell-adhesion properties and good biocompatibility. The present authors recently prepared such a composite by a liquid-phase coating process in which a surface-modified material was immersed in a laminin-containing calcium phosphate solution (LCP solution) supersaturated with respect to apatite. As a result of this coating process, however, the material increased in surface roughness, which may have an adverse effect on the prevention of bacterial infection. In the present study, a reduction of the surface roughness of the laminin-apatite composite layer was attempted by adding MgSO4 to the LCP solution as an inhibitor of apatite crystal growth. The surface roughness, Ra, of the resulting laminin-apatite composite layer decreased from approximately 0.43 to 0.31 μm upon increasing the MgSO4 concentration in the LCP solution from 0 to 1.5 mM, while retaining its cell adhesion characteristics. The thus-obtained material, having a laminin-apatite composite coating with reduced surface roughness, has potential as a material for percutaneous devices with improved resistance to bacterial infection through the interface between the device and the skin.
Original language | English |
---|---|
Pages (from-to) | 1342-1348 |
Number of pages | 7 |
Journal | Acta Biomaterialia |
Volume | 4 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2008 Sep |
Keywords
- Apatite
- Inhibitory effect
- Laminin
- Magnesium
- Percutaneous device
ASJC Scopus subject areas
- Biotechnology
- Biomaterials
- Biochemistry
- Biomedical Engineering
- Molecular Biology