Reduction of surface roughness of a laminin-apatite composite coating via inhibitory effect of magnesium ions on apatite crystal growth

Ayako Oyane, Yuusuke Ishikawa, Atsushi Yamazaki, Yu Sogo, Katsuko Furukawa, Takashi Ushida, Atsuo Ito

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    An artificial material coated with a laminin-apatite composite layer on its surface would be useful as a percutaneous device with cell-adhesion properties and good biocompatibility. The present authors recently prepared such a composite by a liquid-phase coating process in which a surface-modified material was immersed in a laminin-containing calcium phosphate solution (LCP solution) supersaturated with respect to apatite. As a result of this coating process, however, the material increased in surface roughness, which may have an adverse effect on the prevention of bacterial infection. In the present study, a reduction of the surface roughness of the laminin-apatite composite layer was attempted by adding MgSO4 to the LCP solution as an inhibitor of apatite crystal growth. The surface roughness, Ra, of the resulting laminin-apatite composite layer decreased from approximately 0.43 to 0.31 μm upon increasing the MgSO4 concentration in the LCP solution from 0 to 1.5 mM, while retaining its cell adhesion characteristics. The thus-obtained material, having a laminin-apatite composite coating with reduced surface roughness, has potential as a material for percutaneous devices with improved resistance to bacterial infection through the interface between the device and the skin.

    Original languageEnglish
    Pages (from-to)1342-1348
    Number of pages7
    JournalActa Biomaterialia
    Issue number5
    Publication statusPublished - 2008 Sep



    • Apatite
    • Inhibitory effect
    • Laminin
    • Magnesium
    • Percutaneous device

    ASJC Scopus subject areas

    • Biomaterials
    • Biomedical Engineering
    • Biotechnology
    • Biochemistry
    • Molecular Biology

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