Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences

Kazuhiro Furukawa; Hiroshi Abe; Jin Wang; Miwako Uda; Hiroyuki Koshino; Satoshi Tsuneda; Yoshihiro Ito

doi:10.1039/b817228e

Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences

Kazuhiro Furukawa^*, Hiroshi Abe, Jin Wang, Miwako Uda, Hiroyuki Koshino, Satoshi Tsuneda, Yoshihiro Ito

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.

Original language	English
Pages (from-to)	671-677
Number of pages	7
Journal	Organic and Biomolecular Chemistry
Volume	7
Issue number	4
DOIs	https://doi.org/10.1039/b817228e
Publication status	Published - 2009 Feb 16

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

Access to Document

10.1039/b817228e

Cite this

@article{4007e23b8ccc4fe8a64d92ebc2ed6a19,

title = "Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences",

abstract = "We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.",

author = "Kazuhiro Furukawa and Hiroshi Abe and Jin Wang and Miwako Uda and Hiroyuki Koshino and Satoshi Tsuneda and Yoshihiro Ito",

year = "2009",

month = feb,

day = "16",

doi = "10.1039/b817228e",

language = "English",

volume = "7",

pages = "671--677",

journal = "Organic and Biomolecular Chemistry",

issn = "1477-0520",

publisher = "Royal Society of Chemistry",

number = "4",

}

TY - JOUR

T1 - Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences

AU - Furukawa, Kazuhiro

AU - Abe, Hiroshi

AU - Wang, Jin

AU - Uda, Miwako

AU - Koshino, Hiroyuki

AU - Tsuneda, Satoshi

AU - Ito, Yoshihiro

PY - 2009/2/16

Y1 - 2009/2/16

N2 - We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.

AB - We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.

UR - http://www.scopus.com/inward/record.url?scp=59849083263&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59849083263&partnerID=8YFLogxK

U2 - 10.1039/b817228e

DO - 10.1039/b817228e

M3 - Article

C2 - 19194582

AN - SCOPUS:59849083263

SN - 1477-0520

VL - 7

SP - 671

EP - 677

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 4

ER -

Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this