Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences

Kazuhiro Furukawa, Hiroshi Abe, Jin Wang, Miwako Uda, Hiroyuki Koshino, Satoshi Tsuneda, Yoshihiro Ito

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.

    Original languageEnglish
    Pages (from-to)671-677
    Number of pages7
    JournalOrganic and Biomolecular Chemistry
    Volume7
    Issue number4
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    oligonucleotides
    Fluorescent Dyes
    Oligonucleotides
    reagents
    Color
    abl Genes
    color
    leukemias
    Azides
    probes
    nucleotides
    genes
    enzymes
    Leukemia
    Nucleotides
    deoxyribonucleic acid
    Genes
    Fluorescence
    Derivatives
    fluorescence

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry
    • Organic Chemistry
    • Biochemistry

    Cite this

    Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences. / Furukawa, Kazuhiro; Abe, Hiroshi; Wang, Jin; Uda, Miwako; Koshino, Hiroyuki; Tsuneda, Satoshi; Ito, Yoshihiro.

    In: Organic and Biomolecular Chemistry, Vol. 7, No. 4, 2009, p. 671-677.

    Research output: Contribution to journalArticle

    Furukawa, Kazuhiro ; Abe, Hiroshi ; Wang, Jin ; Uda, Miwako ; Koshino, Hiroyuki ; Tsuneda, Satoshi ; Ito, Yoshihiro. / Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences. In: Organic and Biomolecular Chemistry. 2009 ; Vol. 7, No. 4. pp. 671-677.
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