We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.
|Number of pages||7|
|Journal||Organic and Biomolecular Chemistry|
|Publication status||Published - 2009 Feb 16|
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry