Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences

Kazuhiro Furukawa, Hiroshi Abe, Jin Wang, Miwako Uda, Hiroyuki Koshino, Satoshi Tsuneda, Yoshihiro Ito

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37 Citations (Scopus)

Abstract

We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.

Original languageEnglish
Pages (from-to)671-677
Number of pages7
JournalOrganic and Biomolecular Chemistry
Volume7
Issue number4
DOIs
Publication statusPublished - 2009 Feb 16

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ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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