Regeneration of 5-HT fibers in hippocampal heterotopia of methylazoxymethanol-induced micrencephalic rats after neonatal 5,7-DHT injection

Arata Nakamura, Taro Kadowaki, Shinichi Sakakibara, Kanji Yoshimoto, Koichi Hirata, Shuichi Ueda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In order to elucidate the regeneration properties of serotonergic fibers in the hippocampus of methylazoxymethanol acetate (MAM)-induced micrencephalic rats (MAM rats), we examined serotonergic regeneration in the hippocampus following neonatal intracisternal 5,7-dihydroxytryptamine (5,7-DHT) injection. Prenatal exposure to MAM resulted in the formation of hippocampal heterotopia in the dorsal hippocampus. Immunohistochemical and neurochemical analyses revealed hyperinnervation of serotonergic fibers in the hippocampus of MAM rats. After neonatal 5,7-DHT injection, most serotonergic fibers in the hippocampus of 2-week-old MAM rats had degenerated, while a small number of serotonergic fibers in the stratum lacunosum-moleculare (SLM) of the hippocampus and in the hilus adjacent to the granular cell layer of the dentate gyrus (DG) had not. Regenerating serotonergic fibers from the SLM first extended terminals into the hippocampal heterotopia, then fibers from the hilus reinnervated the DG and some fibers extended to the heterotopia. These findings suggest that the hippocampal heterotopia exerts trophic target effects for regenerating serotonergic fibers in the developmental period in micrencephalic rats.

Original languageEnglish
Pages (from-to)38-45
Number of pages8
JournalAnatomical Science International
Volume85
Issue number1
DOIs
Publication statusPublished - 2010 Mar
Externally publishedYes

Fingerprint

5,7-Dihydroxytryptamine
Methylazoxymethanol Acetate
Regeneration
Hippocampus
Serotonin
Injections
Dentate Gyrus
methylazoxymethanol

Keywords

  • Hippocampal heterotopia
  • Methylazoxymethanol acetate
  • Micrencephalic rat
  • Regeneration
  • Serotonergic fibers

ASJC Scopus subject areas

  • Anatomy

Cite this

Regeneration of 5-HT fibers in hippocampal heterotopia of methylazoxymethanol-induced micrencephalic rats after neonatal 5,7-DHT injection. / Nakamura, Arata; Kadowaki, Taro; Sakakibara, Shinichi; Yoshimoto, Kanji; Hirata, Koichi; Ueda, Shuichi.

In: Anatomical Science International, Vol. 85, No. 1, 03.2010, p. 38-45.

Research output: Contribution to journalArticle

Nakamura, Arata ; Kadowaki, Taro ; Sakakibara, Shinichi ; Yoshimoto, Kanji ; Hirata, Koichi ; Ueda, Shuichi. / Regeneration of 5-HT fibers in hippocampal heterotopia of methylazoxymethanol-induced micrencephalic rats after neonatal 5,7-DHT injection. In: Anatomical Science International. 2010 ; Vol. 85, No. 1. pp. 38-45.
@article{65329ad26f4c4dee9e56d0473db2b5ee,
title = "Regeneration of 5-HT fibers in hippocampal heterotopia of methylazoxymethanol-induced micrencephalic rats after neonatal 5,7-DHT injection",
abstract = "In order to elucidate the regeneration properties of serotonergic fibers in the hippocampus of methylazoxymethanol acetate (MAM)-induced micrencephalic rats (MAM rats), we examined serotonergic regeneration in the hippocampus following neonatal intracisternal 5,7-dihydroxytryptamine (5,7-DHT) injection. Prenatal exposure to MAM resulted in the formation of hippocampal heterotopia in the dorsal hippocampus. Immunohistochemical and neurochemical analyses revealed hyperinnervation of serotonergic fibers in the hippocampus of MAM rats. After neonatal 5,7-DHT injection, most serotonergic fibers in the hippocampus of 2-week-old MAM rats had degenerated, while a small number of serotonergic fibers in the stratum lacunosum-moleculare (SLM) of the hippocampus and in the hilus adjacent to the granular cell layer of the dentate gyrus (DG) had not. Regenerating serotonergic fibers from the SLM first extended terminals into the hippocampal heterotopia, then fibers from the hilus reinnervated the DG and some fibers extended to the heterotopia. These findings suggest that the hippocampal heterotopia exerts trophic target effects for regenerating serotonergic fibers in the developmental period in micrencephalic rats.",
keywords = "Hippocampal heterotopia, Methylazoxymethanol acetate, Micrencephalic rat, Regeneration, Serotonergic fibers",
author = "Arata Nakamura and Taro Kadowaki and Shinichi Sakakibara and Kanji Yoshimoto and Koichi Hirata and Shuichi Ueda",
year = "2010",
month = "3",
doi = "10.1007/s12565-009-0052-z",
language = "English",
volume = "85",
pages = "38--45",
journal = "Anatomical Science International",
issn = "1447-6959",
publisher = "American Orthopsychiatric Association Inc.",
number = "1",

}

TY - JOUR

T1 - Regeneration of 5-HT fibers in hippocampal heterotopia of methylazoxymethanol-induced micrencephalic rats after neonatal 5,7-DHT injection

AU - Nakamura, Arata

AU - Kadowaki, Taro

AU - Sakakibara, Shinichi

AU - Yoshimoto, Kanji

AU - Hirata, Koichi

AU - Ueda, Shuichi

PY - 2010/3

Y1 - 2010/3

N2 - In order to elucidate the regeneration properties of serotonergic fibers in the hippocampus of methylazoxymethanol acetate (MAM)-induced micrencephalic rats (MAM rats), we examined serotonergic regeneration in the hippocampus following neonatal intracisternal 5,7-dihydroxytryptamine (5,7-DHT) injection. Prenatal exposure to MAM resulted in the formation of hippocampal heterotopia in the dorsal hippocampus. Immunohistochemical and neurochemical analyses revealed hyperinnervation of serotonergic fibers in the hippocampus of MAM rats. After neonatal 5,7-DHT injection, most serotonergic fibers in the hippocampus of 2-week-old MAM rats had degenerated, while a small number of serotonergic fibers in the stratum lacunosum-moleculare (SLM) of the hippocampus and in the hilus adjacent to the granular cell layer of the dentate gyrus (DG) had not. Regenerating serotonergic fibers from the SLM first extended terminals into the hippocampal heterotopia, then fibers from the hilus reinnervated the DG and some fibers extended to the heterotopia. These findings suggest that the hippocampal heterotopia exerts trophic target effects for regenerating serotonergic fibers in the developmental period in micrencephalic rats.

AB - In order to elucidate the regeneration properties of serotonergic fibers in the hippocampus of methylazoxymethanol acetate (MAM)-induced micrencephalic rats (MAM rats), we examined serotonergic regeneration in the hippocampus following neonatal intracisternal 5,7-dihydroxytryptamine (5,7-DHT) injection. Prenatal exposure to MAM resulted in the formation of hippocampal heterotopia in the dorsal hippocampus. Immunohistochemical and neurochemical analyses revealed hyperinnervation of serotonergic fibers in the hippocampus of MAM rats. After neonatal 5,7-DHT injection, most serotonergic fibers in the hippocampus of 2-week-old MAM rats had degenerated, while a small number of serotonergic fibers in the stratum lacunosum-moleculare (SLM) of the hippocampus and in the hilus adjacent to the granular cell layer of the dentate gyrus (DG) had not. Regenerating serotonergic fibers from the SLM first extended terminals into the hippocampal heterotopia, then fibers from the hilus reinnervated the DG and some fibers extended to the heterotopia. These findings suggest that the hippocampal heterotopia exerts trophic target effects for regenerating serotonergic fibers in the developmental period in micrencephalic rats.

KW - Hippocampal heterotopia

KW - Methylazoxymethanol acetate

KW - Micrencephalic rat

KW - Regeneration

KW - Serotonergic fibers

UR - http://www.scopus.com/inward/record.url?scp=77249159314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77249159314&partnerID=8YFLogxK

U2 - 10.1007/s12565-009-0052-z

DO - 10.1007/s12565-009-0052-z

M3 - Article

VL - 85

SP - 38

EP - 45

JO - Anatomical Science International

JF - Anatomical Science International

SN - 1447-6959

IS - 1

ER -