Region dependent efficiency for recombinational transfer of the Bacillus subtilis 168 genome

Satoshi Tomita, Kenji Tsuge, Yo Kikuchi, Mitsuhiro Itaya

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Submega-sized regions of the Bacillus subtilis genome were cloned to plasmid by the B. subtilis Recombinational Transfer (BReT) method. BReT efficiency depends not only on the genome location but also on the choice of sequences for simultaneous homologous recombination during BReT. In an extreme case, a 91-kb region that was unsuccessful on the first attempt was obtained when the slightly shifted 98-kb region was targeted.

Original languageEnglish
Pages (from-to)1382-1384
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume68
Issue number6
DOIs
Publication statusPublished - 2004 Jun
Externally publishedYes

Keywords

  • Giant plasmid
  • Homologous recombination
  • Landing pad sequence
  • Recombinational transfer

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

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