Regions of unusually high flexibility occur frequently in human genomic DNA

Hajime Kimura, Dai Kageyama, Mika Furuya, Shigeru Sugiyama, Noboru Murata, Takashi Ohyama

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Remarkable progress has been made in genome science during the past decade, but understanding of genomes of eukaryotes is far from complete. We have created DNA flexibility maps of the human, mouse, fruit fly, and nematode chromosomes. The maps revealed that all of these chromosomes have markedly flexible DNA regions (We named them SPIKEs). SPIKEs occur more frequently in the human chromosomes than in the mouse, fruit fly, and nematode chromosomes. Markedly rigid DNA regions (rSPIKEs) are also present in these chromosomes. The ratio of the number of SPIKEs to the total number of SPIKEs and rSPIKEs correlated positively with evolutionary stage among the organisms. Repetitive DNA sequences with flexible and rigid properties contribute to the formation of SPIKEs and rSPIKEs respectively. However, non-repetitive flexible and rigid sequences appear to play a major role in SPIKE and rSPIKE formation respectively. They might be involved in the genome-folding mechanism of eukaryotes.

    Original languageEnglish
    Pages (from-to)612-617
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume77
    Issue number3
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Chromosomes
    DNA
    Genome
    Eukaryota
    Diptera
    Genes
    Fruit
    Fruits
    Nucleic Acid Repetitive Sequences
    Human Chromosomes
    DNA sequences

    Keywords

    • Eukaryotic genome
    • Flexibility map
    • Genome evolution
    • Genome folding
    • Repetitive DNA

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Analytical Chemistry
    • Organic Chemistry

    Cite this

    Regions of unusually high flexibility occur frequently in human genomic DNA. / Kimura, Hajime; Kageyama, Dai; Furuya, Mika; Sugiyama, Shigeru; Murata, Noboru; Ohyama, Takashi.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 77, No. 3, 2013, p. 612-617.

    Research output: Contribution to journalArticle

    Kimura, Hajime ; Kageyama, Dai ; Furuya, Mika ; Sugiyama, Shigeru ; Murata, Noboru ; Ohyama, Takashi. / Regions of unusually high flexibility occur frequently in human genomic DNA. In: Bioscience, Biotechnology and Biochemistry. 2013 ; Vol. 77, No. 3. pp. 612-617.
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