Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

Yuichiro Iwasaki; Kuniki Kino; Hiroyuki Nishide; Kohtaro Kirimura

doi:10.1007/s10529-007-9309-6

Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

Yuichiro Iwasaki, Kuniki Kino, Hiroyuki Nishide, Kohtaro Kirimura^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44% (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.

Original language	English
Pages (from-to)	819-822
Number of pages	4
Journal	Biotechnology Letters
Volume	29
Issue number	5
DOIs	https://doi.org/10.1007/s10529-007-9309-6
Publication status	Published - 2007 May

Keywords

Carboxylation
Resorcinol
Reversible decarboxylase
Rhizobium radiobacter
γ-Resorcylic acid

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-007-9309-6

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title = "Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene",

abstract = "A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44% (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.",

keywords = "Carboxylation, Resorcinol, Reversible decarboxylase, Rhizobium radiobacter, γ-Resorcylic acid",

author = "Yuichiro Iwasaki and Kuniki Kino and Hiroyuki Nishide and Kohtaro Kirimura",

note = "Funding Information: Acknowledgements This work was partly supported by the 21COE program {\textquoteleft}{\textquoteleft}Practical Nano-Chemistry{\textquoteright}{\textquoteright} from MEXT, Japan.",

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doi = "10.1007/s10529-007-9309-6",

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T1 - Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

AU - Iwasaki, Yuichiro

AU - Kino, Kuniki

AU - Nishide, Hiroyuki

AU - Kirimura, Kohtaro

N1 - Funding Information: Acknowledgements This work was partly supported by the 21COE program ‘‘Practical Nano-Chemistry’’ from MEXT, Japan.

PY - 2007/5

Y1 - 2007/5

N2 - A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44% (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.

AB - A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44% (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.

KW - Carboxylation

KW - Resorcinol

KW - Reversible decarboxylase

KW - Rhizobium radiobacter

KW - γ-Resorcylic acid

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JO - Biotechnology Letters

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IS - 5

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Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

Abstract

Keywords

ASJC Scopus subject areas

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