Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

Yuichiro Iwasaki, Kuniki Kino, Hiroyuki Nishide, Kohtaro Kirimura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44% (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.

Original languageEnglish
Pages (from-to)819-822
Number of pages4
JournalBiotechnology Letters
Volume29
Issue number5
DOIs
Publication statusPublished - 2007 May 1

    Fingerprint

Keywords

  • Carboxylation
  • Resorcinol
  • Reversible decarboxylase
  • Rhizobium radiobacter
  • γ-Resorcylic acid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this