Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

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18 Citations (Scopus)

Abstract

A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44% (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.

Original languageEnglish
Pages (from-to)819-822
Number of pages4
JournalBiotechnology Letters
Volume29
Issue number5
DOIs
Publication statusPublished - 2007 May

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Carboxy-Lyases
Escherichia coli
Genes
Cells
Carboxylation
Agrobacterium tumefaciens
Biocatalysts
Acids
Enzymes
resorcinol

Keywords

  • γ-Resorcylic acid
  • Carboxylation
  • Resorcinol
  • Reversible decarboxylase
  • Rhizobium radiobacter

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

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title = "Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene",
abstract = "A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44{\%} (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.",
keywords = "γ-Resorcylic acid, Carboxylation, Resorcinol, Reversible decarboxylase, Rhizobium radiobacter",
author = "Yuichiro Iwasaki and Kuniki Kino and Hiroyuki Nishide and Kotaro Kirimura",
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T1 - Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

AU - Iwasaki, Yuichiro

AU - Kino, Kuniki

AU - Nishide, Hiroyuki

AU - Kirimura, Kotaro

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N2 - A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44% (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.

AB - A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44% (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.

KW - γ-Resorcylic acid

KW - Carboxylation

KW - Resorcinol

KW - Reversible decarboxylase

KW - Rhizobium radiobacter

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